| Literature DB >> 10338212 |
P Gouet1, J M Diprose, J M Grimes, R Malby, J N Burroughs, S Zientara, D I Stuart, P P Mertens.
Abstract
The concentration of double-stranded RNA within the bluetongue virus core renders the genome segments liquid crystalline. Powder diffraction rings confirm this local ordering with a 30 A separation between strands. Determination of the structure of the bluetongue virus core serotype 10 and comparison with that of serotype 1 reveals most of the genomic double-stranded RNA, packaged as well-ordered layers surrounding putative transcription complexes at the apices of the particle. The outer layer of RNA is sufficiently well ordered by interaction with the capsid that a model can be built and extended to the less-ordered inner layers, providing a structural framework for understanding the mechanism of this complex transcriptional machine. We show that the genome segments maintain local order during transcription.Entities:
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Year: 1999 PMID： 10338212 DOI： 10.1016/s0092-8674(00)80758-8
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582