Literature DB >> 10329580

Production and characterization of human monoclonal antibody Fab fragments to vaccinia virus from a phage-display combinatorial library.

C Schmaljohn1, Y Cui, S Kerby, D Pennock, K Spik.   

Abstract

A combinatorial, phage-display library of human Fab antibody fragments was generated from IgG heavy chain (HC) and light chain (LC) genes cloned from the lymphocytes of a vaccinia virus (VACV)-immune donor. To ascertain the complexity of the library, nucleotide sequences of the variable regions of the HC and LC genes were determined. Fourteen distinct HC and 18 distinct LC (7 kappa and 11 lambda) that formed a combinatorial library of 22 Fabs were identified. Immune-precipitation of radiolabeled VACV revealed that at least six different VACV proteins were recognized by the antibodies. Plaque-reduction neutralization demonstrated that six of the Fabs neutralized VACV in the presence of anti-human antibody. ELISA studies indicated that 15 of the Fabs were cross-reactive with monkeypox virus.

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Year:  1999        PMID： 10329580     DOI： 10.1006/viro.1999.9701

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  13 in total

1.  Phage antibodies neutralize vaccinia virus.

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Review 2.  Current and developing technologies for monitoring agents of bioterrorism and biowarfare.

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3.  Structural basis for the binding of the neutralizing antibody, 7D11, to the poxvirus L1 protein.

Authors:  Hua-Poo Su; Joseph W Golden; Apostolos G Gittis; Jay W Hooper; David N Garboczi
Journal:  Virology       Date:  2007-08-03       Impact factor: 3.616

4.  Proteomics-directed cloning of circulating antiviral human monoclonal antibodies.

Authors:  Shuji Sato; Sean A Beausoleil; Lana Popova; Jason G Beaudet; Ravi K Ramenani; Xiaowu Zhang; James S Wieler; Sandra M Schieferl; Wan Cheung Cheung; Roberto D Polakiewicz
Journal:  Nat Biotechnol       Date:  2012-11       Impact factor: 54.908

Review 5.  Bioshock: Biotechnology and Bioterrorism.

Authors:  N Moorchung; A K Sharma; S R Mehta
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6.  Non-coding RNAs and heme oxygenase-1 in vaccinia virus infection.

Authors:  Clement A Meseda; Kumar Srinivasan; Jasen Wise; Jennifer Catalano; Kenneth M Yamada; Subhash Dhawan
Journal:  Biochem Biophys Res Commun       Date:  2014-10-18       Impact factor: 3.575

7.  Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection model.

Authors:  Zhaochun Chen; Patricia Earl; Jeffrey Americo; Inger Damon; Scott K Smith; Fujuan Yu; Andrew Sebrell; Suzanne Emerson; Gary Cohen; Roselyn J Eisenberg; Inna Gorshkova; Peter Schuck; William Satterfield; Bernard Moss; Robert Purcell
Journal:  J Virol       Date:  2007-06-20       Impact factor: 5.103

8.  Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection.

Authors:  N V Tikunova; V V Morozova
Journal:  Acta Naturae       Date:  2009-10       Impact factor: 1.845

9.  A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays antitumor activity against melanoma in vitro and in vivo.

Authors:  Carlos R Figueiredo; Alisson L Matsuo; Ricardo A Azevedo; Mariana H Massaoka; Natalia Girola; Luciano Polonelli; Luiz R Travassos
Journal:  Sci Rep       Date:  2015-09-22       Impact factor: 4.379

Review 10.  The growth and potential of human antiviral monoclonal antibody therapeutics.

Authors:  Wayne A Marasco; Jianhua Sui
Journal:  Nat Biotechnol       Date:  2007-12       Impact factor: 54.908
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