| Literature DB >> 10230039 |
C T Fransen1, K M Van Laere, A A van Wijk, L P Brüll, M Dignum, J E Thomas-Oates, J Haverkamp, H A Schols, A G Voragen, J P Kamerling, J F Vliegenthart.
Abstract
A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)]n-alpha-D-Glcp- (1<-->1)-beta-D-Galp[-(4<--1)-beta-D-Galp]m, with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp- (1<-->1)-beta-D-Galp.Entities:
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Year: 1998 PMID： 10230039 DOI： 10.1016/s0008-6215(98)00285-7
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104