Literature DB >> 10226359

A fast screening method for the identification of siderophores from fluorescent Pseudomonas spp. by liquid chromatography/electrospray mass spectrometry.

S Kilz1, C Lenz, R Fuchs, H Budzikiewicz.   

Abstract

A screening method was developed for the fast identification of known pyoverdin-type siderophores produced by fluorescent Pseudomonas spp. It is based on reversed-phase high-performance liquid chromatography interfaced with electrospray ionization mass spectrometry of the Sep-Pak RP-C18 culture supernatant extracts. The siderophores of five bacterial strains were characterized by their molecular masses obtained from their doubly protonated molecular ions [M + 2H]2+ and their UV/visible spectra recorded with a diode-array detector. Additional structural information was gained by skimmer collision-induced dissociation experiments. For all strains new minor siderophores were found. A table of fully or partially identified pyoverdins and related siderophores is provided which will be the basis for screening studies.

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Year:  1999        PMID： 10226359     DOI： 10.1002/(SICI)1096-9888(199904)34:4<281::AID-JMS750>3.0.CO;2-M

Source DB:  PubMed          Journal:  J Mass Spectrom        ISSN： 1076-5174            Impact factor:   1.982


  7 in total

1.  Infrared multiphoton dissociation of the siderophore enterobactin and its Fe(III) complex. Influence of Fe(III) binding on dissociation kinetics and relative energetics.

Authors:  Andrew D Leslie; Rambod Daneshfar; Dietrich A Volmer
Journal:  J Am Soc Mass Spectrom       Date:  2007-01-05       Impact factor: 3.109

2.  Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes.

Authors:  Haichuan Liu; Kristina Håkansson; Jung Yeop Lee; David H Sherman
Journal:  J Am Soc Mass Spectrom       Date:  2007-02-28       Impact factor: 3.109

3.  Structural characterization and high-throughput screening of inhibitors of PvdQ, an NTN hydrolase involved in pyoverdine synthesis.

Authors:  Eric J Drake; Andrew M Gulick
Journal:  ACS Chem Biol       Date:  2011-09-15       Impact factor: 5.100

4.  High-performance liquid chromatography analyses of pyoverdin siderophores differentiate among phytopathogenic fluorescent Pseudomonas Species.

Authors:  Alain Bultreys; Isabelle Gheysen; Bernard Wathelet; Henri Maraite; Edmond de Hoffmann
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792

5.  Paerucumarin, a new metabolite produced by the pvc gene cluster from Pseudomonas aeruginosa.

Authors:  Michael F Clarke-Pearson; Sean F Brady
Journal:  J Bacteriol       Date:  2008-08-08       Impact factor: 3.490

6.  An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides.

Authors:  Matthew C T Hartman; Kristopher Josephson; Chi-Wang Lin; Jack W Szostak
Journal:  PLoS One       Date:  2007-10-03       Impact factor: 3.240

7.  Draft Genome Sequence of Pseudomonas fluorescens LMG 5329, a White Line-Inducing Principle-Producing Bioindicator for the Mushroom Pathogen Pseudomonas tolaasii.

Authors:  Maarten G K Ghequire; Hassan Rokni-Zadeh; Peyman Zarrineh; René De Mot
Journal:  Genome Announc       Date:  2013-07-25
  7 in total

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