Literature DB >> 10222598

Electrospray mass spectra from protein electroeluted from sodium dodecylsulfate polyacrylamide gel electrophoresis gels.

E K Fridriksson1, B Baird, F W McLafferty.   

Abstract

Electrospray ionization/tandem mass spectrometry of proteins separated on sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels is severely limited by the requirement that the protein be completely separated from the SDS. As shown here, the gaseous noncovalent SDS adducts of protonated proteins thus formed can be dissociated by infrared irradiation. ESI spectra from myoglobin in SDS-containing solutions show molecular ions adducted with up to 15 dodecyl sulfates, but ir irradiation of these ions causes complete dissociation to expel the SDS.

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Year:  1999        PMID： 10222598     DOI： 10.1016/S1044-0305(99)00012-4

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  15 in total

1.  Infrared photodissociation of non-covalent adducts of electrosprayed nucleotide ions.

Authors:  D P Little; F W McLafferty
Journal:  J Am Soc Mass Spectrom       Date:  1996-02       Impact factor: 3.109

2.  Capillary column high-performance liquid chromatographic-electrospray ionization triple-stage quadrupole mass spectrometric analysis of proteins separated by two-dimensional polyacrylamide gel electrophoresis. Application to cerebellar protein mapping.

Authors:  H Nakayama; K Uchida; F Shinkai; T Shinoda; T Okuyama; K Seta; T Isobe
Journal:  J Chromatogr A       Date:  1996-04-12       Impact factor: 4.759

3.  Mass spectrometry of proteins directly from polyacrylamide gels.

Authors:  R R Ogorzalek Loo; T I Stevenson; C Mitchell; J A Loo; P C Andrews
Journal:  Anal Chem       Date:  1996-06-01       Impact factor: 6.986

4.  Analytical properties of the nanoelectrospray ion source.

Authors:  M Wilm; M Mann
Journal:  Anal Chem       Date:  1996-01-01       Impact factor: 6.986

5.  Isoforms of a cuticular protein from larvae of the meal beetle, Tenebrio molitor, studied by mass spectrometry in combination with Edman degradation and two-dimensional polyacrylamide gel electrophoresis.

Authors:  S Haebel; C Jensen; S O Andersen; P Roepstorff
Journal:  Protein Sci       Date:  1995-03       Impact factor: 6.725

6.  Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.

Authors:  M Wilm; A Shevchenko; T Houthaeve; S Breit; L Schweigerer; T Fotsis; M Mann
Journal:  Nature       Date:  1996-02-01       Impact factor: 49.962

Review 7.  Mass spectrometric approaches for the identification of gel-separated proteins.

Authors:  S D Patterson; R Aebersold
Journal:  Electrophoresis       Date:  1995-10       Impact factor: 3.535

8.  Sequence verification of human creatine kinase (43 kDa) isozymes by high-resolution tandem mass spectrometry.

Authors:  T D Wood; L H Chen; C B White; P C Babbitt; G L Kenyon; F W McLafferty
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

9.  Thiaminase I (42 kDa) heterogeneity, sequence refinement, and active site location from high-resolution tandem mass spectrometry.

Authors:  N L Kelleher; C A Costello; T P Begley; F W McLafferty
Journal:  J Am Soc Mass Spectrom       Date:  1995-10       Impact factor: 3.109

10.  Infrared multiphoton dissociation of large multiply charged ions for biomolecule sequencing.

Authors:  D P Little; J P Speir; M W Senko; P B O'Connor; F W McLafferty
Journal:  Anal Chem       Date:  1994-09-15       Impact factor: 6.986
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  6 in total

1.  Competitive binding to the oligopeptide binding protein, OppA: in-trap cleanup in an Fourier transform ion cyclotron resonance mass spectrometer.

Authors:  M A Freitas; C L Hendrickson; A G Marshall; A A Rostom; C V Robinson
Journal:  J Am Soc Mass Spectrom       Date:  2000-11       Impact factor: 3.109

2.  Regulation of perforin activation and pre-synaptic toxicity through C-terminal glycosylation.

Authors:  Imran G House; Colin M House; Amelia J Brennan; Omer Gilan; Mark A Dawson; James C Whisstock; Ruby Hp Law; Joseph A Trapani; Ilia Voskoboinik
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Review 3.  Comprehensive analysis of protein modifications by top-down mass spectrometry.

Authors:  Han Zhang; Ying Ge
Journal:  Circ Cardiovasc Genet       Date:  2011-12

4.  Collision-induced dissociation of bradykinin ions in the interface region of an ESI-MS.

Authors:  B B Schneider; D J Douglas; D D Chen
Journal:  J Am Soc Mass Spectrom       Date:  2001-07       Impact factor: 3.262

5.  Empirical evaluation of bone extraction protocols.

Authors:  Timothy P Cleland; Kristyn Voegele; Mary H Schweitzer
Journal:  PLoS One       Date:  2012-02-14       Impact factor: 3.240

6.  Bone protein "extractomics": comparing the efficiency of bone protein extractions of Gallus gallus in tandem mass spectrometry, with an eye towards paleoproteomics.

Authors:  Elena R Schroeter; Caroline J DeHart; Mary H Schweitzer; Paul M Thomas; Neil L Kelleher
Journal:  PeerJ       Date:  2016-10-27       Impact factor: 2.984
  6 in total

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