BACKGROUND: A cytogenetic study of 75 consecutive children with ALL revealed a normal karyotype, a low hyperdiploid karyotype (including 47-50 chromosomes), and a high hyperdiploid karyotype (including > 50 chromosomes) in 10, 12, and 33 patients, respectively. An acquired extra X-chromosome was detected at diagnosis by conventional cytogenetics in 29 (88%) of 33 children with a high hyperdiploid karyotype and in 4 (33%) of 12 children with a low hyperdiploid karyotype. X-chromosome aneuploidy was retrospectively studied by fluorescence in situ hybridization (FISH) in eight and 20 patients with a normal and a hyperdiploid karyotype, respectively. PROCEDURE: A classical cytogenetic study was performed according to standard methods. FISH with the centromeric probe specific to X-chromosome was used to study interphase cells of bone marrow or blood samples. RESULTS: An extra X-chromosome was found by FISH in all 13 patients with a high hyperdiploid or tetraploid, in 6 of 7 patients with a low hyperdiploid, and in none with a normal karyotype. Two children with a normal karyotype displayed monosomy X. Altogether, 57.3% of newly diagnosed children displayed X-chromosome aneuploidy. CONCLUSIONS: Out study indicates that X-chromosome aneuploidy may be the most common chromosome abnormality in childhood ALL. It can be detected in nearly all children with a high hyperdiploid karyotype and up to one-half of the patients with a low hyperdiploid karyotype. FISH with an X-chromosome centromeric probe is a rapid and simple tool to detect an abnormal clone at diagnosis in the majority of children with ALL and is useful in confirming remission in these patients.
BACKGROUND: A cytogenetic study of 75 consecutive children with ALL revealed a normal karyotype, a low hyperdiploid karyotype (including 47-50 chromosomes), and a high hyperdiploid karyotype (including > 50 chromosomes) in 10, 12, and 33 patients, respectively. An acquired extra X-chromosome was detected at diagnosis by conventional cytogenetics in 29 (88%) of 33 children with a high hyperdiploid karyotype and in 4 (33%) of 12 children with a low hyperdiploid karyotype. X-chromosome aneuploidy was retrospectively studied by fluorescence in situ hybridization (FISH) in eight and 20 patients with a normal and a hyperdiploid karyotype, respectively. PROCEDURE: A classical cytogenetic study was performed according to standard methods. FISH with the centromeric probe specific to X-chromosome was used to study interphase cells of bone marrow or blood samples. RESULTS: An extra X-chromosome was found by FISH in all 13 patients with a high hyperdiploid or tetraploid, in 6 of 7 patients with a low hyperdiploid, and in none with a normal karyotype. Two children with a normal karyotype displayed monosomy X. Altogether, 57.3% of newly diagnosed children displayed X-chromosome aneuploidy. CONCLUSIONS: Out study indicates that X-chromosome aneuploidy may be the most common chromosome abnormality in childhood ALL. It can be detected in nearly all children with a high hyperdiploid karyotype and up to one-half of the patients with a low hyperdiploid karyotype. FISH with an X-chromosome centromeric probe is a rapid and simple tool to detect an abnormal clone at diagnosis in the majority of children with ALL and is useful in confirming remission in these patients.
Authors: Takeshi Isoda; Anthony M Ford; Daisuke Tomizawa; Frederik W van Delft; David Gonzalez De Castro; Norkio Mitsuiki; Joannah Score; Tomohiko Taki; Tomohiro Morio; Masatoshi Takagi; Hiroh Saji; Mel Greaves; Shuki Mizutani Journal: Proc Natl Acad Sci U S A Date: 2009-10-12 Impact factor: 11.205
Authors: Florian A Karreth; Markus Reschke; Anna Ruocco; Christopher Ng; Bjoern Chapuy; Valentine Léopold; Marcela Sjoberg; Thomas M Keane; Akanksha Verma; Ugo Ala; Yvonne Tay; David Wu; Nina Seitzer; Martin Del Castillo Velasco-Herrera; Anne Bothmer; Jacqueline Fung; Fernanda Langellotto; Scott J Rodig; Olivier Elemento; Margaret A Shipp; David J Adams; Roberto Chiarle; Pier Paolo Pandolfi Journal: Cell Date: 2015-04-02 Impact factor: 41.582
Authors: Miroslav Djokic; Elisabet Björklund; Elisabeth Blennow; Joanna Mazur; Stefan Söderhäll; Anna Porwit Journal: Haematologica Date: 2009-05-19 Impact factor: 9.941
Authors: Eda Yildirim; James E Kirby; Diane E Brown; Francois E Mercier; Ruslan I Sadreyev; David T Scadden; Jeannie T Lee Journal: Cell Date: 2013-02-14 Impact factor: 41.582