The location of active site opening and closing events in the prehydride transfer phase of the oxidative deamination reaction catalyzed by bovine liver glutamate dehydrogenase using a novel pH jump approach.

A pH jump approach has been developed and used to locate the opening and closing events occurring in the time course of the beef liver glutamate dehydrogenase catalyzed reaction. A comparison of the pH jump results, the resolved component time courses, and the 340 nm fluorescence signal suggests the existence and location on the reaction time course of a previously unreported prehydride transfer complex.