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Literature DB >> 10209136

Modifying secretion and post-translational processing in insect cells.

Abstract

The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.

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Year: 1999 PMID： 10209136 DOI： 10.1016/s0958-1669(99)80024-x

Source DB: PubMed Journal: Curr Opin Biotechnol ISSN： 0958-1669 Impact factor: 9.740

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Cited

16 in total

1. A bi-cistronic baculovirus expression vector for improved recombinant protein production.

Authors: Tzong-Yuan Wu; Ying-Ju Chen; Chao-Yi Teng; Wen-Shuo Chen; Oliver Villaflores
Journal: Bioeng Bugs Date: 2012-03-01

2. Design of an efficient medium for insect cell growth and recombinant protein production.

Authors: L Ikonomou; G Bastin; Y J Schneider; S N Agathos
Journal: In Vitro Cell Dev Biol Anim Date: 2001-10 Impact factor: 2.416

3. Recombinant soluble betaglycan is a potent and isoform-selective transforming growth factor-beta neutralizing agent.

Authors: M M Vilchis-Landeros; J L Montiel; V Mendoza; G Mendoza-Hernández; F López-Casillas
Journal: Biochem J Date: 2001-04-01 Impact factor: 3.857

4. Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system.

Authors: V A Morais; J Serpa; A S Palma; T Costa; L Maranga; J Costa
Journal: Biochem J Date: 2001-02-01 Impact factor: 3.857

Modifying secretion and post-translational processing in insect cells.

1. A bi-cistronic baculovirus expression vector for improved recombinant protein production.

2. Design of an efficient medium for insect cell growth and recombinant protein production.

3. Recombinant soluble betaglycan is a potent and isoform-selective transforming growth factor-beta neutralizing agent.

4. Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system.

5. Dot-Blotting: A Quick Method for Expression Analysis of Recombinant Proteins.

6. Enhancement of correct protein folding in vivo by a non-lytic baculovirus.

Review 7. Baculovirus as versatile vectors for protein expression in insect and mammalian cells.

8. The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi.

9. Expression of recombinant antibodies.

10. Production of a recombinant antibody fragment in whole insect larvae.