| Literature DB >> 10198428 |
T Fukagawa1, N Hayward, J Yang, C Azzalin, D Griffin, A F Stewart, W Brown.
Abstract
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.Entities:
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Year: 1999 PMID： 10198428 PMCID： PMC148408 DOI： 10.1093/nar/27.9.1966
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971