OBJECTIVE: The aim of this study was to determine whether lectin binding to exfoliated human colonocytes could be used as a noninvasive test for colorectal polyps or cancer. METHODS: Colonocytes were harvested from 31 patients (10 controls, 10 with adenomatous polyps, and 11 with cancer), incubated with a panel of fluorescent-labeled lectins, and assayed by flow cytometry. RESULTS: The lectins jacalin (JAC) and wheat germ agglutinin (WGA) were useful in predicting the presence of a colorectal neoplasm (p = 0.0018 for JAC and p = 0.0099 for WGA). For JAC, sensitivity reached 81% with a specificity of 80%, and for WGA the sensitivity and specificity were both 75%. CONCLUSIONS: Lectin binding to human colonocytes can predict the presence of malignant and premalignant lesions of the colon, and has potential as a noninvasive screening tool for colorectal neoplasms.
OBJECTIVE: The aim of this study was to determine whether lectin binding to exfoliated human colonocytes could be used as a noninvasive test for colorectal polyps or cancer. METHODS: Colonocytes were harvested from 31 patients (10 controls, 10 with adenomatous polyps, and 11 with cancer), incubated with a panel of fluorescent-labeled lectins, and assayed by flow cytometry. RESULTS: The lectins jacalin (JAC) and wheat germ agglutinin (WGA) were useful in predicting the presence of a colorectal neoplasm (p = 0.0018 for JAC and p = 0.0099 for WGA). For JAC, sensitivity reached 81% with a specificity of 80%, and for WGA the sensitivity and specificity were both 75%. CONCLUSIONS: Lectin binding to human colonocytes can predict the presence of malignant and premalignant lesions of the colon, and has potential as a noninvasive screening tool for colorectal neoplasms.
Authors: H Rasinperä; C Forsblom; N S Enattah; P Halonen; K Salo; M Victorzon; J-P Mecklin; H Järvinen; S Enholm; G Sellick; H Alazzouzi; R Houlston; J Robinson; P-H Groop; I Tomlinson; S Schwartz; L A Aaltonen; I Järvelä Journal: Gut Date: 2005-05 Impact factor: 23.059
Authors: Ying Jie Yu; Adhip P N Majumdar; Jordan M Nechvatal; Jeffrey L Ram; Marc D Basson; Lance K Heilbrun; Ikuko Kato Journal: Cancer Epidemiol Biomarkers Prev Date: 2008-02 Impact factor: 4.254
Authors: Ikuko Kato; Kawsar Z Badsha; Susan Land; Jordan M Nechvatal; Larry H Matherly; Adi L Tarca; Adhip P Majumdar; Marc D Basson; Jeffrey L Ram Journal: Tumori Date: 2009 Nov-Dec
Authors: Joe Chin-Hun Kuo; Ashraf E K Ibrahim; Sarah Dawson; Deepak Parashar; William J Howat; Kiran Guttula; Richard Miller; Nicola S Fearnhead; Douglas J Winton; André A Neves; Kevin M Brindle Journal: Sci Rep Date: 2016-04-13 Impact factor: 4.379