Literature DB >> 10085057

Biological effects of Pseudomonas aeruginosa type III-secreted proteins on CHO cells.

A J Vallis1, V Finck-Barbançon, T L Yahr, D W Frank.   

Abstract

A strain of Pseudomonas aeruginosa that fails to express known type III-secreted effector proteins was constructed as an expression host. Individual effectors were expressed in trans, and their biological effects on CHO cells were assessed in an acute cellular infection model. Intoxication with ExoS, ExoT, or ExoY resulted in alterations in cell morphology. As shown in previous genetic studies, ExoU expression was linked to acute cytotoxicity.

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Year:  1999        PMID： 10085057      PMCID： PMC96567          DOI： 10.1128/IAI.67.4.2040-2044.1999

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  22 in total

1.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

2.  Pseudomonas aeruginosa exoenzyme S: an adenosine diphosphate ribosyltransferase distinct from toxin A.

Authors:  B H Iglewski; J Sadoff; M J Bjorn; E S Maxwell
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

3.  ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injury.

Authors:  V Finck-Barbançon; J Goranson; L Zhu; T Sawa; J P Wiener-Kronish; S M Fleiszig; C Wu; L Mende-Mueller; D W Frank
Journal:  Mol Microbiol       Date:  1997-08       Impact factor: 3.501

4.  Intracellular expression of the ADP-ribosyltransferase domain of Pseudomonas exoenzyme S is cytotoxic to eukaryotic cells.

Authors:  K J Pederson; J T Barbieri
Journal:  Mol Microbiol       Date:  1998-11       Impact factor: 3.501

5.  Induction of a novel morphological response in Chinese hamster ovary cells by pertussis toxin.

Authors:  E L Hewlett; K T Sauer; G A Myers; J L Cowell; R L Guerrant
Journal:  Infect Immun       Date:  1983-06       Impact factor: 3.441

6.  Intracellular targeting of exoenzyme S of Pseudomonas aeruginosa via type III-dependent translocation induces phagocytosis resistance, cytotoxicity and disruption of actin microfilaments.

Authors:  E Frithz-Lindsten; Y Du; R Rosqvist; A Forsberg
Journal:  Mol Microbiol       Date:  1997-09       Impact factor: 3.501

7.  Identification and characterization of SpcU, a chaperone required for efficient secretion of the ExoU cytotoxin.

Authors:  V Finck-Barbançon; T L Yahr; D W Frank
Journal:  J Bacteriol       Date:  1998-12       Impact factor: 3.490

8.  Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.

Authors:  F Denizot; R Lang
Journal:  J Immunol Methods       Date:  1986-05-22       Impact factor: 2.303

9.  ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system.

Authors:  T L Yahr; A J Vallis; M K Hancock; J T Barbieri; D W Frank
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-10       Impact factor: 11.205

10.  Regulation of ExoS production and secretion by Pseudomonas aeruginosa in response to tissue culture conditions.

Authors:  A J Vallis; T L Yahr; J T Barbieri; D W Frank
Journal:  Infect Immun       Date:  1999-02       Impact factor: 3.441
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  66 in total

1.  Characterization of a Hank's type serine/threonine kinase and serine/threonine phosphoprotein phosphatase in Pseudomonas aeruginosa.

Authors:  S Mukhopadhyay; V Kapatral; W Xu; A M Chakrabarty
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

2.  Pseudomonas aeruginosa ExoT is a Rho GTPase-activating protein.

Authors:  R Krall; G Schmidt; K Aktories; J T Barbieri
Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

3.  Traversal of multilayered corneal epithelia by cytotoxic Pseudomonas aeruginosa requires the phospholipase domain of exoU.

Authors:  Julio C Ramirez; Suzanne M J Fleiszig; Aaron B Sullivan; Connie Tam; Roya Borazjani; David J Evans
Journal:  Invest Ophthalmol Vis Sci       Date:  2012-01-25       Impact factor: 4.799

4.  Role of the membrane localization domain of the Pseudomonas aeruginosa effector protein ExoU in cytotoxicity.

Authors:  Jeff L Veesenmeyer; Heather Howell; Andrei S Halavaty; Sebastian Ahrens; Wayne F Anderson; Alan R Hauser
Journal:  Infect Immun       Date:  2010-05-17       Impact factor: 3.441

5.  Type 3 Secretion Translocators Spontaneously Assemble a Hexadecameric Transmembrane Complex.

Authors:  Fabian B Romano; Yuzhou Tang; Kyle C Rossi; Kathryn R Monopoli; Jennifer L Ross; Alejandro P Heuck
Journal:  J Biol Chem       Date:  2016-01-19       Impact factor: 5.157

6.  Examination of the coordinate effects of Pseudomonas aeruginosa ExoS on Rac1.

Authors:  Claudia L Rocha; Elizabeth A Rucks; Deanne M Vincent; Joan C Olson
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

7.  The ADP ribosyltransferase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities.

Authors:  L Garrity-Ryan; S Shafikhani; P Balachandran; L Nguyen; J Oza; T Jakobsen; J Sargent; X Fang; S Cordwell; M A Matthay; J N Engel
Journal:  Infect Immun       Date:  2004-01       Impact factor: 3.441

8.  In vitro assays to monitor the activity of Pseudomonas aeruginosa Type III secreted proteins.

Authors:  Stephanie L Rolsma; Dara W Frank
Journal:  Methods Mol Biol       Date:  2014

9.  Pseudomonas aeruginosa induces localized immunosuppression during pneumonia.

Authors:  Maureen H Diaz; Ciara M Shaver; John D King; Srinidhi Musunuri; Jeffrey A Kazzaz; Alan R Hauser
Journal:  Infect Immun       Date:  2008-07-28       Impact factor: 3.441

10.  A sensitive fluorescence-based assay for the detection of ExoU-mediated PLA(2) activity.

Authors:  Marc A Benson; Katherine M Schmalzer; Dara W Frank
Journal:  Clin Chim Acta       Date:  2009-11-10       Impact factor: 3.786
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