Literature DB >> 10082383

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis.

C Kinsland1, S V Taylor, N L Kelleher, F W McLafferty, T P Begley.   

Abstract

A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

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Year:  1998        PMID： 10082383      PMCID： PMC2144084          DOI： 10.1002/pro.5560070821

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  12 in total

Review 1.  Protein splicing and autoproteolysis mechanisms.

Authors:  F B Perler; M Q Xu; H Paulus
Journal:  Curr Opin Chem Biol       Date:  1997-10       Impact factor: 8.822

2.  Analytical properties of the nanoelectrospray ion source.

Authors:  M Wilm; M Mann
Journal:  Anal Chem       Date:  1996-01-01       Impact factor: 6.986

Review 3.  Protein synthesis by chemical ligation of unprotected peptides in aqueous solution.

Authors:  T W Muir; P E Dawson; S B Kent
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

4.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

5.  Protein splicing: estimation of the rate of O-N and S-N acyl rearrangements, the last step of the splicing process.

Authors:  Y Shao; H Paulus
Journal:  J Pept Res       Date:  1997-09

6.  Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.

Authors:  P B Vander Horn; A D Backstrom; V Stewart; T P Begley
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

7.  Protein splicing: characterization of the aminosuccinimide residue at the carboxyl terminus of the excised intervening sequence.

Authors:  Y Shao; M Q Xu; H Paulus
Journal:  Biochemistry       Date:  1995-08-29       Impact factor: 3.162

8.  Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation.

Authors:  S V Taylor; N L Kelleher; C Kinsland; H J Chiu; C A Costello; A D Backstrom; F W McLafferty; T P Begley
Journal:  J Biol Chem       Date:  1998-06-26       Impact factor: 5.157

9.  Peptide synthesis using unprotected peptides through orthogonal coupling methods.

Authors:  J P Tam; Y A Lu; C F Liu; J Shao
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-19       Impact factor: 11.205

10.  Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation.

Authors:  M Q Xu; D G Comb; H Paulus; C J Noren; Y Shao; F B Perler
Journal:  EMBO J       Date:  1994-12-01       Impact factor: 11.598
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  14 in total

1.  InBase, the Intein Database.

Authors:  F B Perler
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

2.  Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

Authors:  J Xi; Y Ge; C Kinsland; F W McLafferty; T P Begley
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-03       Impact factor: 11.205

3.  Reagent for the detection of protein thiocarboxylates in the bacterial proteome: lissamine rhodamine B sulfonyl azide.

Authors:  Kalyanaraman Krishnamoorthy; Tadhg P Begley
Journal:  J Am Chem Soc       Date:  2010-08-25       Impact factor: 15.419

4.  Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier.

Authors:  Annemarthe G Van der Veen; Kenji Schorpp; Christian Schlieker; Ludovico Buti; Jadyn R Damon; Eric Spooner; Hidde L Ploegh; Stefan Jentsch
Journal:  Proc Natl Acad Sci U S A       Date:  2011-01-05       Impact factor: 11.205

Review 5.  Thiamin biosynthesis: still yielding fascinating biological chemistry.

Authors:  Tadhg P Begley; Steven E Ealick; Fred W McLafferty
Journal:  Biochem Soc Trans       Date:  2012-06-01       Impact factor: 5.407

6.  Protein thiocarboxylate-dependent methionine biosynthesis in Wolinella succinogenes.

Authors:  Kalyanaraman Krishnamoorthy; Tadhg P Begley
Journal:  J Am Chem Soc       Date:  2010-12-16       Impact factor: 15.419

7.  Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA.

Authors:  Minghao Chen; Shin-Ichi Asai; Shun Narai; Shusuke Nambu; Naoki Omura; Yuriko Sakaguchi; Tsutomu Suzuki; Masao Ikeda-Saito; Kimitsuna Watanabe; Min Yao; Naoki Shigi; Yoshikazu Tanaka
Journal:  Proc Natl Acad Sci U S A       Date:  2017-04-24       Impact factor: 11.205

8.  Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium tuberculosis.

Authors:  Kristin E Burns; Sabine Baumgart; Pieter C Dorrestein; Huili Zhai; Fred W McLafferty; Tadhg P Begley
Journal:  J Am Chem Soc       Date:  2005-08-24       Impact factor: 15.419

9.  Investigation of the C-terminal redox center of high-Mr thioredoxin reductase by protein engineering and semisynthesis.

Authors:  Brian E Eckenroth; Brian M Lacey; Adam P Lothrop; Katharine M Harris; Robert J Hondal
Journal:  Biochemistry       Date:  2007-07-28       Impact factor: 3.162

10.  O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.

Authors:  Seán E O'Leary; Christopher T Jurgenson; Steven E Ealick; Tadhg P Begley
Journal:  Biochemistry       Date:  2008-10-09       Impact factor: 3.162
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