Literature DB >> 10075882

Monitoring mRNA decapping activity.

S Zhang1, C J Williams, M Wormington, A Stevens, S W Peltz.   

Abstract

mRNA decapping is a common step shared between two important mRNA decay pathways in yeast, Saccharomyces cerevisiae. To investigate how mRNAs are decapped, we have developed an assay that can be easily used to measure the decapping activity. This assay has been used to isolate yeast strains with altered decapping activities. The results demonstrated that decreased decapping activity in vitro corresponds well with the decapping-deficient phenotype in vivo. This assay has been applied to the purified yeast decapping enzyme Dcp1 protein as well as crude yeast extracts and Xenopus oocyte extracts. Copyright 1999 Academic Press.

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Year:  1999        PMID： 10075882     DOI： 10.1006/meth.1998.0706

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  36 in total

1.  Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay.

Authors:  Jens Lykke-Andersen
Journal:  Mol Cell Biol       Date:  2002-12       Impact factor: 4.272

Review 2.  MRNA stability and the control of gene expression: implications for human disease.

Authors:  Elysia M Hollams; Keith M Giles; Andrew M Thomson; Peter J Leedman
Journal:  Neurochem Res       Date:  2002-10       Impact factor: 3.996

3.  The protein Zfand5 binds and stabilizes mRNAs with AU-rich elements in their 3'-untranslated regions.

Authors:  Guoan He; Dongxu Sun; Zhiying Ou; Aihao Ding
Journal:  J Biol Chem       Date:  2012-06-04       Impact factor: 5.157

4.  Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.

Authors:  Leah S Cohen; Claudette Mikhli; Cassandra Friedman; Marzena Jankowska-Anyszka; Janusz Stepinski; Edward Darzynkiewicz; Richard E Davis
Journal:  RNA       Date:  2004-10       Impact factor: 4.942

5.  The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer.

Authors:  Nicola Minshall; Nancy Standart
Journal:  Nucleic Acids Res       Date:  2004-02-24       Impact factor: 16.971

6.  Role of p54 RNA helicase activity and its C-terminal domain in translational repression, P-body localization and assembly.

Authors:  Nicola Minshall; Michel Kress; Dominique Weil; Nancy Standart
Journal:  Mol Biol Cell       Date:  2009-03-18       Impact factor: 4.138

7.  An unconventional human Ccr4-Caf1 deadenylase complex in nuclear cajal bodies.

Authors:  Eileen Wagner; Sandra L Clement; Jens Lykke-Andersen
Journal:  Mol Cell Biol       Date:  2006-12-18       Impact factor: 4.272

8.  Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe.

Authors:  Meipei She; Carolyn J Decker; Nan Chen; Suneeta Tumati; Roy Parker; Haiwei Song
Journal:  Nat Struct Mol Biol       Date:  2005-12-11       Impact factor: 15.369

9.  Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts.

Authors:  Joseph Milone; Jeffrey Wilusz; Vivian Bellofatto
Journal:  Nucleic Acids Res       Date:  2002-09-15       Impact factor: 16.971

10.  Analysis of the products of mRNA decapping and 3'-to-5' decay by denaturing gel electrophoresis.

Authors:  Naomi Bergman; Mateusz Opyrchal; Elizabeth J Bates; Jeffrey Wilusz
Journal:  RNA       Date:  2002-07       Impact factor: 4.942
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