Arthritogenicity of Yersinia enterocolitica O:8 in hamsters: analysis of the immune response.

An animal model, hamster, was used for the study of Yersinia-induced arthritis. The development of arthritis, estimated by measuring the inflammation on hind paws after infection, was correlated with the kinetics of the immune response. Histological and immunofluorescence (IFI) studies and serum antibody measurements were performed. Two inflammatory peaks were observed: an acute one on day 11 post-infection (p.i.) and a chronic one on days 26-35 p.i. Joint cultures were positive until day 14 p.i. IFI was used to demonstrate the deposit of bacterial antigens in the joint. A persistent response of cellular extract-specific IgG antibodies was observed until day 94. Lipopolysaccharide-specific IgG was statistically significant on day 26 p.i. Antibodies against bands 66 and 54 were observed by immunoblotting. Polyclonal activation was detected during reactive arthritis. It is shown that Y. enterocolitica is arthritogenic in hamsters, immune mechanisms participating in the development of this disease.