Literature DB >> 10048336

Is human thioredoxin monomeric or dimeric?

A M Gronenborn1, G M Clore, J M Louis, P T Wingfield.   

Abstract

We have examined the molecular weight and rotational correlation time of human thioredoxin by analytical ultracentrifugation and NMR spectroscopy, respectively. Two variants of human thioredoxin were studied, namely human thioredoxin identical in amino acid sequence to the one whose NMR structure we previously determined (C62A, C69A, C73A, M74T) and human thioredoxin (C62A, C69A, C73A, M74) containing the wild-type amino acid methionine at position 74. In both cases, the experimental data indicate that the predominant species is monomeric and we find no evidence for the existence of a well-defined dimeric form as was observed in the recently reported crystal structure (Weichsel et al., 1996) of human thioredoxin and the C73S mutant.

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Year:  1999        PMID： 10048336      PMCID： PMC2144256          DOI： 10.1110/ps.8.2.426

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  9 in total

1.  Analysis of the backbone dynamics of interleukin-1 beta using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy.

Authors:  G M Clore; P C Driscoll; P T Wingfield; A M Gronenborn
Journal:  Biochemistry       Date:  1990-08-14       Impact factor: 3.162

2.  The extracellular domain of immunodeficiency virus gp41 protein: expression in Escherichia coli, purification, and crystallization.

Authors:  P T Wingfield; S J Stahl; J Kaufman; A Zlotnick; C C Hyde; A M Gronenborn; G M Clore
Journal:  Protein Sci       Date:  1997-08       Impact factor: 6.725

3.  Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer.

Authors:  A Weichsel; J R Gasdaska; G Powis; W R Montfort
Journal:  Structure       Date:  1996-06-15       Impact factor: 5.006

4.  Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields.

Authors:  N Tjandra; P Wingfield; S Stahl; A Bax
Journal:  J Biomol NMR       Date:  1996-10       Impact factor: 2.835

5.  Analysis of the backbone dynamics of interleukin-8 by 15N relaxation measurements.

Authors:  B L Grasberger; A M Gronenborn; G M Clore
Journal:  J Mol Biol       Date:  1993-03-20       Impact factor: 5.469

6.  Human thioredoxin homodimers: regulation by pH, role of aspartate 60, and crystal structure of the aspartate 60 --> asparagine mutant.

Authors:  J F Andersen; D A Sanders; J R Gasdaska; A Weichsel; G Powis; W R Montfort
Journal:  Biochemistry       Date:  1997-11-18       Impact factor: 3.162

7.  The high-resolution three-dimensional solution structures of the oxidized and reduced states of human thioredoxin.

Authors:  J Qin; G M Clore; A M Gronenborn
Journal:  Structure       Date:  1994-06-15       Impact factor: 5.006

8.  1H and 15N resonance assignments and secondary structure of the human thioredoxin C62A, C69A, C73A mutant.

Authors:  J D Forman-Kay; G M Clore; S J Stahl; A M Gronenborn
Journal:  J Biomol NMR       Date:  1992-09       Impact factor: 2.835

9.  Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease.

Authors:  L E Kay; D A Torchia; A Bax
Journal:  Biochemistry       Date:  1989-11-14       Impact factor: 3.162
  9 in total
  6 in total

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Authors:  Amit Kumar; Priscilla Ward; Uma V Katre; Smita Mohanty
Journal:  Biopolymers       Date:  2012-02-03       Impact factor: 2.505

2.  Structural Mechanisms of Mutant Huntingtin Aggregation Suppression by the Synthetic Chaperonin-like CCT5 Complex Explained by Cryoelectron Tomography.

Authors:  Michele C Darrow; Oksana A Sergeeva; Jose M Isas; Jesús G Galaz-Montoya; Jonathan A King; Ralf Langen; Michael F Schmid; Wah Chiu
Journal:  J Biol Chem       Date:  2015-05-20       Impact factor: 5.157

3.  Crystal structure of human thioredoxin revealing an unraveled helix and exposed S-nitrosation site.

Authors:  Andrzej Weichsel; Michelle Kem; William R Montfort
Journal:  Protein Sci       Date:  2010-09       Impact factor: 6.725

4.  The conformational stability and biophysical properties of the eukaryotic thioredoxins of Pisum sativum are not family-conserved.

Authors:  David Aguado-Llera; Ana Isabel Martínez-Gómez; Jesús Prieto; Marco Marenchino; José Angel Traverso; Javier Gómez; Ana Chueca; José L Neira
Journal:  PLoS One       Date:  2011-02-22       Impact factor: 3.240

5.  Chaperone-like properties of tobacco plastid thioredoxins f and m.

Authors:  Ruth Sanz-Barrio; Alicia Fernández-San Millán; Jon Carballeda; Patricia Corral-Martínez; José M Seguí-Simarro; Inmaculada Farran
Journal:  J Exp Bot       Date:  2011-09-23       Impact factor: 6.992

6.  Crystallographic studies evidencing the high energy tolerance to disrupting the interface disulfide bond of thioredoxin 1 from white leg shrimp Litopenaeus vannamei.

Authors:  Adam A Campos-Acevedo; Enrique Rudiño-Piñera
Journal:  Molecules       Date:  2014-12-15       Impact factor: 4.411
  6 in total

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