Literature DB >> 10026257

Reconstitution of functional 50S ribosomes from in vitro transcripts of Bacillus stearothermophilus 23S rRNA.

R Green1, H F Noller.   

Abstract

In vitro transcripts of Bacillus stearothermophilus 23S rRNA can be reconstituted into catalytically active 50S ribosomal subunits with an efficiency only 3-4-fold lower than that of natural 23S rRNA. Thus, post-transcriptional modifications in 23S rRNA are not essential for the assembly or function of the 50S subunit of the ribosome. This reconstitution sytem has been used to characterize the peptidyl transferase activity of site-directed mutations in 23S rRNA at positions G2252, U2506, U2584, and A2602 (Escherichia coli numbering), demonstrating its potential for the analysis of the role played by 23S rRNA in the function of the 50S subunit of the ribosome.

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Year:  1999        PMID： 10026257     DOI： 10.1021/bi982246a

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  39 in total

1.  Peptidyl transferase antibiotics perturb the relative positioning of the 3'-terminal adenosine of P/P'-site-bound tRNA and 23S rRNA in the ribosome.

Authors:  S V Kirillov; B T Porse; R A Garrett
Journal:  RNA       Date:  1999-08       Impact factor: 4.942

Review 2.  Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae.

Authors:  D Kressler; P Linder; J de La Cruz
Journal:  Mol Cell Biol       Date:  1999-12       Impact factor: 4.272

3.  Posttranscriptional modifications in the A-loop of 23S rRNAs from selected archaea and eubacteria.

Authors:  M A Hansen; F Kirpekar; W Ritterbusch; B Vester
Journal:  RNA       Date:  2002-02       Impact factor: 4.942

4.  Ribosomal protein L5 has a highly twisted concave surface and flexible arms responsible for rRNA binding.

Authors:  T Nakashima; M Yao; S Kawamura; K Iwasaki; M Kimura; I Tanaka
Journal:  RNA       Date:  2001-05       Impact factor: 4.942

Review 5.  After the ribosome structures: how are the subunits assembled?

Authors:  James R Williamson
Journal:  RNA       Date:  2003-02       Impact factor: 4.942

6.  RNA structure comparison, motif search and discovery using a reduced representation of RNA conformational space.

Authors:  Carlos M Duarte; Leven M Wadley; Anna Marie Pyle
Journal:  Nucleic Acids Res       Date:  2003-08-15       Impact factor: 16.971

7.  Interference probing of rRNA with snoRNPs: a novel approach for functional mapping of RNA in vivo.

Authors:  Ben Liu; Maurille J Fournier
Journal:  RNA       Date:  2004-07       Impact factor: 4.942

8.  Isolation of antibiotic resistance mutations in the rRNA by using an in vitro selection system.

Authors:  Luisa Cochella; Rachel Green
Journal:  Proc Natl Acad Sci U S A       Date:  2004-03-04       Impact factor: 11.205

9.  Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants.

Authors:  Kenji Nishimura; Shanna K Johansen; Takashi Inaoka; Takeshi Hosaka; Shinji Tokuyama; Yasutaka Tahara; Susumu Okamoto; Fujio Kawamura; Stephen Douthwaite; Kozo Ochi
Journal:  J Bacteriol       Date:  2007-06-15       Impact factor: 3.490

10.  Exploration of the conserved A+C wobble pair within the ribosomal peptidyl transferase center using affinity purified mutant ribosomes.

Authors:  Ashley Eversole Hesslein; Vladimir I Katunin; Malte Beringer; Anne B Kosek; Marina V Rodnina; Scott A Strobel
Journal:  Nucleic Acids Res       Date:  2004-07-15       Impact factor: 16.971
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