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Literature DB >> 10021399

Directed evolution of biocatalysts.

Abstract

Directed evolution is being used increasingly in academic and industrial laboratories to modify and improve important biocatalysts. Significant advances during this period of review include compartmentalization of genes and the in vitro translation apparatus in emulsions, as well as several impressive demonstrations of catalyst improvement. Shuffling of homologous genes offers a new way to utilize natural diversity in the evolution of novel catalysts.

Mesh：

Substances：
Enzymes

Year: 1999 PMID： 10021399 DOI： 10.1016/s1367-5931(99)80010-6

Source DB: PubMed Journal: Curr Opin Chem Biol ISSN： 1367-5931 Impact factor: 8.822

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Cited

49 in total

1. Understanding thermostability in cytochrome P450 by combinatorial mutagenesis.

Authors: S A Maves; S G Sligar
Journal: Protein Sci Date: 2001-01 Impact factor: 6.725

2. Sialidase-like Asp-boxes: sequence-similar structures within different protein folds.

Authors: R R Copley; R B Russell; C P Ponting
Journal: Protein Sci Date: 2001-02 Impact factor: 6.725

3. Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants.

Authors: Y S Kim; H C Jung; J G Pan
Journal: Appl Environ Microbiol Date: 2000-02 Impact factor: 4.792

4. Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways.

Authors: C Jürgens; A Strom; D Wegener; S Hettwer; M Wilmanns; R Sterner
Journal: Proc Natl Acad Sci U S A Date: 2000-08-29 Impact factor: 11.205

Directed evolution of biocatalysts.

1. Understanding thermostability in cytochrome P450 by combinatorial mutagenesis.

2. Sialidase-like Asp-boxes: sequence-similar structures within different protein folds.

3. Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants.

4. Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways.

5. Enzyme-like proteins by computational design.

6. The generality of DNA-templated synthesis as a basis for evolving non-natural small molecules.

Review 7. Improving the quality of industrially important enzymes by directed evolution.

8. eCodonOpt: a systematic computational framework for optimizing codon usage in directed evolution experiments.

9. Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates.

10. Prospecting metagenomic enzyme subfamily genes for DNA family shuffling by a novel PCR-based approach.