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Literature DB >> 11693193

Improving the quality of industrially important enzymes by directed evolution.

R R Chirumamilla¹, R Muralidhar, R Marchant, P Nigam.

Abstract

Directed evolution is a new process for developing industrially viable biocatalysts. This technique does not require a comprehensive knowledge of the relationships between sequence structure and function of proteins as required by protein engineering. It mimics the process of Darwinian evolution in a test tube combining random mutagenesis and recombination with screening or selection for enzyme variants that have the desired properties. Directed evolution helps in enhancing the enzyme performance both in natural and synthetic environments. This article reviews the process of directed evolution and its application to improve substrate specificity, activity, enantioselectivity and thermal stability.

Mesh：

Substances：

Year: 2001 PMID： 11693193 DOI： 10.1023/a:1011904405002

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

61 in total

1. Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation.

Authors: H Joo; Z Lin; F H Arnold
Journal: Nature Date: 1999-06-17 Impact factor: 49.962

2. Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold.

Authors: M M Altamirano; J M Blackburn; C Aguayo; A R Fersht
Journal: Nature Date: 2000-02-10 Impact factor: 49.962

3. Evolution in the test tube as a means to create enantioselective enzymes for use in organic synthesis.

Authors: M T Reetz
Journal: Sci Prog Date: 2000 Impact factor: 2.774

4. Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues.

Authors: S Oue; A Okamoto; T Yano; H Kagamiyama
Journal: J Biol Chem Date: 1999-01-22 Impact factor: 5.157

5. DNA shuffling of a family of genes from diverse species accelerates directed evolution.

Authors: A Crameri; S A Raillard; E Bermudez; W P Stemmer
Journal: Nature Date: 1998-01-15 Impact factor: 49.962

6. Directed evolution of an aspartate aminotransferase with new substrate specificities.

Authors: T Yano; S Oue; H Kagamiyama
Journal: Proc Natl Acad Sci U S A Date: 1998-05-12 Impact factor: 11.205

7. Cloning of random-sequence oligodeoxynucleotides.

Authors: A R Oliphant; A L Nussbaum; K Struhl
Journal: Gene Date: 1986 Impact factor: 3.688

8. Structural comparisons of TIM barrel proteins suggest functional and evolutionary relationships between beta-galactosidase and other glycohydrolases.

Authors: D H Juers; R E Huber; B W Matthews
Journal: Protein Sci Date: 1999-01 Impact factor: 6.725

9. Functional expression of horseradish peroxidase in E. coli by directed evolution.

Authors: Z Lin; T Thorsen; F H Arnold
Journal: Biotechnol Prog Date: 1999 May-Jun

10. Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay.

Authors: E Henke; U T Bornscheuer
Journal: Biol Chem Date: 1999 Jul-Aug Impact factor: 3.915

6 in total

1. Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR.

Authors: Yilin Le; Huayou Chen; Robert Zagursky; J H David Wu; Weilan Shao
Journal: DNA Res Date: 2013-04-30 Impact factor: 4.458

6. Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis Isolates as Potential Producers of Thermostable Enzymes.

Authors: Balsam T Mohammad; Hala I Al Daghistani; Atef Jaouani; Saleh Abdel-Latif; Christian Kennes
Journal: Int J Microbiol Date: 2017-10-15