| Literature DB >> 9986860 |
P Chanbancherd1, A E Brown, R Trichavaroj, P Tienamporn, P Puthakird, N Limpairojn, T C VanCott, M S de Souza.
Abstract
Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.Entities:
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Year: 1999 PMID: 9986860 PMCID: PMC84563
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948