| Literature DB >> 9972542 |
K Kigasawa1, H Ishikawa, H Obazawa, T Minamoto, Y Nagai, Y Tanaka.
Abstract
We investigated extracellular matrix produced by human retinal pigment epithelial cells (RPE) in vitro using electron microscopy and enzyme linked immunosorbent assay (ELISA). The thickness of the matrix under the cell layer was about 30 microns after 360 days of culture. It consisted mainly of fibrous and granular components. Type IV and V collagen were detected but type I and III were not detected by ELISA. It appeared that RPE can secrete type IV and V collagen and form a thick membrane which may cause proliferative vitreoretinopathy (PVR) by contraction. Control of RPE proliferation and secretion of extracellular matrix is indispensable for prevention of PVR.Entities:
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Year: 1998 PMID： 9972542
Source DB: PubMed Journal: Tokai J Exp Clin Med ISSN： 0385-0005