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Literature DB >> 995642

Circular dichroism as a probe of DNA structure inside reconstituted nucleohistones.

A Garel, A M Kovacs, M Champagne, M Daune.

Abstract

Reconstituted nucleohistones were obtained by mixing in given conditions acid extracted histones and eukaryotic DNA. The histone/DNA ratio (w/w) was in the range 0.35 - 0.95. With the four histones (H2A2B) we have been able to obtain subunits (nucleosomes or upsilon-bodies). The variation of cirsular dichroism signal with temperature at 280 nm was measured to follow structural changes of the DNA inside the complex. The true change of ellipticity (see article) of histone-bound DNA regions, is similar for reconstituted nucleohistone and H1-depleted chromatin, and is therefore a physical probe of the presence of nucleosomes.

Entities: Chemical

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Year: 1976 PMID： 995642 PMCID： PMC343109 DOI： 10.1093/nar/3.10.2507

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

Circular dichroism as a probe of DNA structure inside reconstituted nucleohistones.

1. Studies on chromatin. II. Isolation and characterization of chromatin subunits.

2. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

3. Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt.

4. The subunit structure of the eukaryotic chromosome.

5. Comparison between histones FV and F2a2 of chicken erythrocyte. II. Interaction with homologous DNA.

6. Subunit structure of chromatin is the same in plants and animals.

7. Chromatin fragments resembling v bodies.

8. Release of discrete subunits after nuclease and trypsin digestion of chromatin.

9. Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils.

10. Protein measurement with the Folin phenol reagent.

1. Physical properties of inner histone-DNA complexes.

2. Reconstitution of chromatin: assembly of the nucleosome.

3. Changes in the circular dichroic spectrum of calf thymus solubilized chromatin caused by ultraviolet irradiation.

4. Core nucleosomes by digestion of reconstructed histone-DNA complexes.