Literature DB >> 9952442

Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores.

K Higuchi1, K Suzuki, H Nakanishi, H Yamaguchi, N K Nishizawa, S Mori.   

Abstract

Nicotianamine synthase (NAS), the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores, catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine. We purified NAS protein and isolated the genes nas1, nas2, nas3, nas4, nas5-1, nas5-2, and nas6, which encode NAS and NAS-like proteins from Fe-deficient barley (Hordeum vulgare L. cv Ehimehadaka no. 1) roots. Escherichia coli expressing nas1 showed NAS activity, confirming that this gene encodes a functional NAS. Expression of nas genes as determined by northern-blot analysis was induced by Fe deficiency and was root specific. The NAS genes form a multigene family in the barley and rice genomes.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 9952442      PMCID： PMC32123          DOI： 10.1104/pp.119.2.471

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  14 in total

1.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

2.  Sodium dodecyl sulfate-gel electrophoresis: staining of polypeptides using heavy metal salts.

Authors:  J K Dzandu; J F Johnson; G E Wise
Journal:  Anal Biochem       Date:  1988-10       Impact factor: 3.365

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Evidence for a specific uptake system for iron phytosiderophores in roots of grasses.

Authors:  V Römheld; H Marschner
Journal:  Plant Physiol       Date:  1986-01       Impact factor: 8.340

6.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

7.  Biosynthesis of Phytosiderophores : In Vitro Biosynthesis of 2'-Deoxymugineic Acid from l-Methionine and Nicotianamine.

Authors:  S Shojima; N K Nishizawa; S Fushiya; S Nozoe; T Irifune; S Mori
Journal:  Plant Physiol       Date:  1990-08       Impact factor: 8.340

8.  Two Related Biosynthetic Pathways of Mugineic Acids in Gramineous Plants.

Authors:  J. F. Ma; K. Nomoto
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

9.  Polyamine synthesis in mammalian tissues. Isolation and characterization of spermine synthase from bovine brain.

Authors:  R L Pajula; A Raina; T Eloranta
Journal:  Eur J Biochem       Date:  1979-11

10.  Differential expression of conglycinin α' and β subunit genes in transgenic plants.

Authors:  S Naito; P H Dubé; R N Beachy
Journal:  Plant Mol Biol       Date:  1988-03       Impact factor: 4.076
View more
  91 in total

1.  Map-based cloning of chloronerva, a gene involved in iron uptake of higher plants encoding nicotianamine synthase.

Authors:  H Q Ling; G Koch; H Bäumlein; M W Ganal
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-08       Impact factor: 11.205

2.  Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize.

Authors:  Mikio Nakazono; Fang Qiu; Lisa A Borsuk; Patrick S Schnable
Journal:  Plant Cell       Date:  2003-03       Impact factor: 11.277

3.  An integrated approach for the comparative analysis of a multigene family: the nicotianamine synthase genes of barley.

Authors:  Dragan Perovic; Peter Tiffin; Dimitar Douchkov; Helmut Bäumlein; Andreas Graner
Journal:  Funct Integr Genomics       Date:  2007-01-11       Impact factor: 3.410

4.  Expression, purification, crystallization and preliminary X-ray analysis of an archaeal protein homologous to plant nicotianamine synthase.

Authors:  Cyril Dreyfus; David Pignol; Pascal Arnoux
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-09-30

5.  Mutations in Arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds.

Authors:  Brian M Waters; Heng-Hsuan Chu; Raymond J Didonato; Louis A Roberts; Robynn B Eisley; Brett Lahner; David E Salt; Elsbeth L Walker
Journal:  Plant Physiol       Date:  2006-06-30       Impact factor: 8.340

Review 6.  Iron homeostasis and plant immune responses: Recent insights and translational implications.

Authors:  John H Herlihy; Terri A Long; John M McDowell
Journal:  J Biol Chem       Date:  2020-07-30       Impact factor: 5.157

7.  Expression and enzyme activity of glutathione reductase is upregulated by Fe-deficiency in graminaceous plants.

Authors:  Khurram Bashir; Seiji Nagasaka; Reiko Nakanishi Itai; Takanori Kobayashi; Michiko Takahashi; Hiromi Nakanishi; Satoshi Mori; Naoko K Nishizawa
Journal:  Plant Mol Biol       Date:  2007-08-21       Impact factor: 4.076

8.  FRD3, a member of the multidrug and toxin efflux family, controls iron deficiency responses in Arabidopsis.

Authors:  Elizabeth E Rogers; Mary Lou Guerinot
Journal:  Plant Cell       Date:  2002-08       Impact factor: 11.277

Review 9.  The molecular mechanism of zinc and cadmium stress response in plants.

Authors:  Ya-Fen Lin; Mark G M Aarts
Journal:  Cell Mol Life Sci       Date:  2012-08-18       Impact factor: 9.261

10.  Mutation in nicotianamine aminotransferase stimulated the Fe(II) acquisition system and led to iron accumulation in rice.

Authors:  Longjun Cheng; Fang Wang; Huixia Shou; Fangliang Huang; Luqing Zheng; Fei He; Jinhui Li; Fang-Jie Zhao; Daisei Ueno; Jian Feng Ma; Ping Wu
Journal:  Plant Physiol       Date:  2007-10-19       Impact factor: 8.340
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.