Literature DB >> 99480

Estimation of antibodies specific for dextran.

L Matsuuchi, S L Morrison.   

Abstract

Methods are described for the isolation and characterization of picogram quantities of anti-dextran antibodies. 14C-dextrans produced by using the dextransucrases of Leuconostoc mesenteroides strains B1355 and B512 were used in a radioimmunoassay. The specificity of this assay was verified by using cell cytoplasmic lysates from mouse plasmacytomas, J558 (anti-alpha 1 leads to 3 dextran) and W3129 (anti-alpha 1 leads to 6 dextran). Dextran produced by strain B1355 and insolubilized with epichlorohydrin was used as an immunoabsorbent.

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Year:  1978        PMID： 99480

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  4 in total

1.  Antigen size and charge in immune complex glomerulonephritis. II. Passive induction of immune deposits with dextran-anti-dextran immune complexes.

Authors:  K L Isaacs; F Miller
Journal:  Am J Pathol       Date:  1983-06       Impact factor: 4.307

2.  Isolation and characterization of a variant of mouse plasmacytoma J558 synthesizing a 110,000-dalton immunoglobulin heavy chain and of secondary variants synthesizing either a 55,000-dalton or an 80,000-dalton immunoglobulin heavy chain: possible implications.

Authors:  L Matsuuchi; S L Morrison
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272

3.  Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies.

Authors:  J Sharon; S L Morrison; E A Kabat
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

4.  Glycosylation of a VH residue of a monoclonal antibody against alpha (1----6) dextran increases its affinity for antigen.

Authors:  S C Wallick; E A Kabat; S L Morrison
Journal:  J Exp Med       Date:  1988-09-01       Impact factor: 14.307
  4 in total

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