C H Holschneider1, F Nejad, F J Montz. 1. Department of Obstetrics and Gynecology, University of California, Los Angeles, School of Medicine, USA.
Abstract
OBJECTIVE: To assess the effects of two macrophage down-regulating cytokines (interleukin [IL]-10 and IL-4) and the nonsteroidal anti-inflammatory drug ketorolac tromethamine on postoperative adhesion formation. DESIGN: Randomized controlled trial. SETTING: Research center vivarium. ANIMAL(S): Six-week-old Swiss Webster mice. INTERVENTION(S): One hundred eighty animals were randomized to serve as nonsurgical controls or to undergo a standardized adhesion-inducing procedure. Subsequently, animals were randomized to nine different treatment groups to receive no injections, phosphate-buffered saline (PBS) only, IL-10, IL-4, ketorolac, IL-10 plus IL-4, IL-10 plus ketorolac, IL-4 plus ketorolac, or all three agents. MAIN OUTCOME MEASURE: Adhesion scores on postoperative day 10. RESULT(S): Postoperative adhesion scores were significantly reduced in all groups of animals treated with IL-10 or ketorolac. Animals treated with IL-4 showed a nonsignificant trend toward reduction of adhesions. There were significantly more animals with adhesion scores of < or = 3 in the IL-10 and ketorolac treatment groups than in the control groups receiving no treatment or PBS only. CONCLUSION(S): Although treatment with IL-10 and ketorolac did not completely prevent adhesion formation, treatment with these drugs did lead to a significant reduction in adhesion formation. Adhesions also tended to be thin and filmy rather than thick and vascular. Addition of IL-4 did not augment these effects.
OBJECTIVE: To assess the effects of two macrophage down-regulating cytokines (interleukin [IL]-10 and IL-4) and the nonsteroidal anti-inflammatory drug ketorolac tromethamine on postoperative adhesion formation. DESIGN: Randomized controlled trial. SETTING: Research center vivarium. ANIMAL(S): Six-week-old Swiss Webster mice. INTERVENTION(S): One hundred eighty animals were randomized to serve as nonsurgical controls or to undergo a standardized adhesion-inducing procedure. Subsequently, animals were randomized to nine different treatment groups to receive no injections, phosphate-buffered saline (PBS) only, IL-10, IL-4, ketorolac, IL-10 plus IL-4, IL-10 plus ketorolac, IL-4 plus ketorolac, or all three agents. MAIN OUTCOME MEASURE: Adhesion scores on postoperative day 10. RESULT(S): Postoperative adhesion scores were significantly reduced in all groups of animals treated with IL-10 or ketorolac. Animals treated with IL-4 showed a nonsignificant trend toward reduction of adhesions. There were significantly more animals with adhesion scores of < or = 3 in the IL-10 and ketorolac treatment groups than in the control groups receiving no treatment or PBS only. CONCLUSION(S): Although treatment with IL-10 and ketorolac did not completely prevent adhesion formation, treatment with these drugs did lead to a significant reduction in adhesion formation. Adhesions also tended to be thin and filmy rather than thick and vascular. Addition of IL-4 did not augment these effects.
Authors: Begonia Ruiz-Perez; Doo R Chung; Arlene H Sharpe; Hideo Yagita; Wiltrud M Kalka-Moll; Mohamed H Sayegh; Dennis L Kasper; Arthur O Tzianabos Journal: Proc Natl Acad Sci U S A Date: 2005-11-07 Impact factor: 11.205
Authors: Anja Hirschelmann; Garri Tchartchian; Markus Wallwiener; Andreas Hackethal; Rudy Leon De Wilde Journal: Arch Gynecol Obstet Date: 2011-10-30 Impact factor: 2.344