| Literature DB >> 9932795 |
T Gerken1, J Kurtz, K P Sauer, T Lubjuhn.
Abstract
A simple and time-saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1-5 microL of insect hemolymph in a Chelex 100-suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.Entities:
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Year: 1998 PMID： 9932795 DOI： 10.1002/elps.1150191804
Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535