Literature DB >> 993070

The histochemical demonstration of choline acetyltransferase by semipermeable membrane technique.

G Hüther, H Luppa.   

Abstract

The application of the semipermeable membrane technique in light microscopical demonstration of choline acetyltransferase is described. The method founds upon earlier developed lead salt techniques. Use of semipermeable membranes fully prevents any loss of enzyme by dissolvement or inactivation during fixation. Addition of NaCl to the incubation medium markedly increases the activity of choline acetyltransferase.

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Year:  1976        PMID： 993070     DOI： 10.1007/bf00496133

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  12 in total

1.  Histochemical methods for acid beta-galactosidase: technics for semipermeable membranes.

Authors:  Z Lojda
Journal:  Histochemie       Date:  1973-12-31

Review 2.  Molecular properties of choline acetyltransferase and their importance for the compartmentation of acetylcholine synthesis.

Authors:  F Fonnum; D Malthe-Sorenssen
Journal:  Prog Brain Res       Date:  1972       Impact factor: 2.453

3.  Choline acetyltransferase and acetylcholinesterase in spinal motor neurons cultured in vitro.

Authors:  S U Kim
Journal:  Experientia       Date:  1972-05-15

4.  Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. I. Acid phosphatase.

Authors:  A E Meijer
Journal:  Histochemie       Date:  1972

5.  Localization of choline acetyltransferase.

Authors:  P Kása; S P Mann; C Hebb
Journal:  Nature       Date:  1970-05-30       Impact factor: 49.962

6.  The effect of a hemisection on the distribution of acetylcholinesterase and choline acetyltransferase in the spinal cord of the cat.

Authors:  D G Gwyn; J H Wolstencroft; A Silver
Journal:  Brain Res       Date:  1972-12-12       Impact factor: 3.252

7.  Antibodies to rat brain choline acetyltransferase: species and organ specificity.

Authors:  J Rossier; A Bauman; P Benda
Journal:  FEBS Lett       Date:  1973-10-01       Impact factor: 4.124

8.  Choline acetyltransferase localization in the central nervous system by immunohistochemistry.

Authors:  P L McGeer; E G McGeer; V K Singh; W H Chase
Journal:  Brain Res       Date:  1974-12-06       Impact factor: 3.252

Review 9.  Neurotransmitter synthetic enzymes.

Authors:  P L McGeer; E G McGeer
Journal:  Prog Neurobiol       Date:  1973       Impact factor: 11.685

10.  Modified microassay for the rapid and sensitive determination of choline acetyltransferase activity using (3H)-acetyl-CoA and Fonnum's extraction.

Authors:  V Bigl
Journal:  Acta Biol Med Ger       Date:  1975
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  2 in total

1.  The multiple forms of brain acetylcholinesterase. I. Micro-electrophoresis and topochemical analysis of the pattern.

Authors:  G Hüther; H Luppa
Journal:  Histochemistry       Date:  1977-09-22

Review 2.  The histochemistry of carboxylester hydrolases: problems and possibilities.

Authors:  H Luppa; J Andrä
Journal:  Histochem J       Date:  1983-02
  2 in total

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