Literature DB >> 993061

An X-ray microanalytical azo dye technique for the localization of acid phosphatase activity.

I D Bowen, T A Ryder, N L Downing.   

Abstract

A new method is described for the histochemical localization of acid phosphatase. Naphthol AS BI, enzymatically released from naphthyl AS BI phosphoric acid, is coupled with diazotized 2,5-dibromoaniline to produce a fine insoluble red azo dye. The histochemical and cytochemical localization of this final reaction product in rat liver is described. In the electron microscope, sites of the azo dye can be detected by X-ray microanalysis of ultrathin cryosections of reactive tissue.

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Year:  1976        PMID： 993061     DOI： 10.1007/bf00490125

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  16 in total

Review 1.  THE LOCALIZATION OF PHOSPHATASE ACTIVITIES AT THE LEVEL OF ULTRASTRUCTURE.

Authors:  S GOLDFISCHER; E ESSNER; A B NOVIKOFF
Journal:  J Histochem Cytochem       Date:  1964-02       Impact factor: 2.479

Review 2.  LOCALIZATION OF ENZYMATIC ACTIVITY AT THE FINE STRUCTURAL LEVEL.

Authors:  R J BARRNETT
Journal:  J R Microsc Soc       Date:  1964-06

3.  New diazonium components as coupling agents in the demonstration of phosphatases.

Authors:  M S BURSTONE; E K WEISBURGER
Journal:  J Histochem Cytochem       Date:  1961-05       Impact factor: 2.479

4.  Dual localization of beta-glucuronidase in endoplasmic reticulum and in lysosomes.

Authors:  W H Fishman; S S Goldman; R DeLellis
Journal:  Nature       Date:  1967-02-04       Impact factor: 49.962

5.  A high resolution technique for the fine-structural localization of acid hydrolases.

Authors:  I D Bowden
Journal:  J Microsc       Date:  1971-08       Impact factor: 1.758

6.  Electron-cytochemical localization of acid phosphatase activity in the digestive caeca of the desert locust.

Authors:  I D Bowen
Journal:  J R Microsc Soc       Date:  1968

7.  Acid hydrolases in the suckling rat small intestine. II. On the importance of alkaline phosphatase inhibition in the histochemical localization of acid phosphatase activity.

Authors:  M J Connock; A P Sturdee
Journal:  Histochem J       Date:  1975-03

8.  The importance of osmiophilia in the production of stable azoindoxyl complexes of high contrast for combined enzyme cytochemistry and electron microscopy.

Authors:  S J Holt; R M Hicks
Journal:  J Cell Biol       Date:  1966-05       Impact factor: 10.539

9.  DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY.

Authors:  L W TICE; R J BARRNETT
Journal:  J Cell Biol       Date:  1965-04       Impact factor: 10.539

10.  Combined cytochemical and electron microscopic demonstration of beta-glucuronidase activity in rat liver with the use of a simultaneous coupling azo dye technique.

Authors:  M Hayashi; T Shirahama; A S Cohen
Journal:  J Cell Biol       Date:  1968-02       Impact factor: 10.539
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  3 in total

Review 1.  X-ray microanalysis: a histochemical tool for elemental analysis.

Authors:  A T Sumner
Journal:  Histochem J       Date:  1983-06

2.  The localization of histochemical and autoradiographic products in the scanning electron microscope by means of atomic number contrast.

Authors:  I D Bowen; G H Lewis; C Winters
Journal:  Histochem J       Date:  1983-12

3.  A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry.

Authors:  G H Lewis; I D Bowen
Journal:  Histochem J       Date:  1985-04
  3 in total

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