Literature DB >> 9928998

A recombinant single-chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector.

M Mousli1, C Devaux, H Rochat, M Goyffon, P Billiald.   

Abstract

Monoclonal antibody 4C1 specifically binds to and neutralizes the most potent neurotoxin (AahII) of the scorpion Androctonus australis. The cDNAs encoding the variable regions of this antibody were isolated by PCR-mediated cloning. A single-chain Fv gene was engineered and expressed in Escherichia coli. The recombinant protein had neutralizing activity similar to that of the intact antibody in vitro and in vivo. We have thus neutralized the pharmacological and biological properties of a scorpion neurotoxin with a single-chain Fv, which opens new perspectives for the treatment of envenomizations.

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Year:  1999        PMID： 9928998     DOI： 10.1016/s0014-5793(98)01647-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  12 in total

1.  Diabody mixture providing full protection against experimental scorpion envenoming with crude Androctonus australis venom.

Authors:  Anne di Tommaso; Matthieu O Juste; Marie-France Martin-Eauclaire; Isabelle Dimier-Poisson; Philippe Billiald; Nicolas Aubrey
Journal:  J Biol Chem       Date:  2012-02-28       Impact factor: 5.157

2.  Structural insights into antibody sequestering and neutralizing of Na+ channel α-type modulator from old world scorpion venom.

Authors:  Igor P Fabrichny; Grégoire Mondielli; Sandrine Conrod; Marie-France Martin-Eauclaire; Yves Bourne; Pascale Marchot
Journal:  J Biol Chem       Date:  2012-02-27       Impact factor: 5.157

3.  Exploiting cross-reactivity to neutralize two different scorpion venoms with one single chain antibody fragment.

Authors:  Lidia Riaño-Umbarila; Gabriel Contreras-Ferrat; Timoteo Olamendi-Portugal; Citlalli Morelos-Juárez; Gerardo Corzo; Lourival D Possani; Baltazar Becerril
Journal:  J Biol Chem       Date:  2010-12-14       Impact factor: 5.157

4.  In vivo neutralization of botulinum neurotoxins serotype E with heavy-chain camelid antibodies (VHH).

Authors:  Hamid Bakherad; Seyed Latif Mousavi Gargari; Iraj Rasooli; Masoumeh Rajabibazl; Mohammad Mohammadi; Walead Ebrahimizadeh; Leila Safaee Ardakani; Hamed Zare
Journal:  Mol Biotechnol       Date:  2013-10       Impact factor: 2.695

5.  Different expression systems resulted in varied binding properties of anti-paclitaxel single-chain variable fragment antibody clone 1C2.

Authors:  Gorawit Yusakul; Seiichi Sakamoto; Poomraphie Nuntawong; Hiroyuki Tanaka; Satoshi Morimoto
Journal:  J Nat Med       Date:  2017-10-12       Impact factor: 2.343

Review 6.  Engineering venom's toxin-neutralizing antibody fragments and its therapeutic potential.

Authors:  Larissa M Alvarenga; Muhammad Zahid; Anne di Tommaso; Matthieu O Juste; Nicolas Aubrey; Philippe Billiald; Julien Muzard
Journal:  Toxins (Basel)       Date:  2014-08-21       Impact factor: 4.546

7.  A method to confer Protein L binding ability to any antibody fragment.

Authors:  Zineb Lakhrif; Martine Pugnière; Corinne Henriquet; Anne di Tommaso; Isabelle Dimier-Poisson; Philippe Billiald; Matthieu O Juste; Nicolas Aubrey
Journal:  MAbs       Date:  2015-12-18       Impact factor: 5.857

Review 8.  Serotherapy against Voltage-Gated Sodium Channel-Targeting αToxins from Androctonus Scorpion Venom.

Authors:  Marie-France Martin-Eauclaire; Sonia Adi-Bessalem; Djelila Hammoudi-Triki; Fatima Laraba-Djebari; Pierre E Bougis
Journal:  Toxins (Basel)       Date:  2019-01-23       Impact factor: 4.546

9.  Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.

Authors:  Nidiane D R Prado; Soraya S Pereira; Michele P da Silva; Michelle S S Morais; Anderson M Kayano; Leandro S Moreira-Dill; Marcos B Luiz; Fernando B Zanchi; André L Fuly; Maribel E F Huacca; Cleberson F Fernandes; Leonardo A Calderon; Juliana P Zuliani; Luiz H Pereira da Silva; Andreimar M Soares; Rodrigo G Stabeli; Carla F C Fernandes
Journal:  PLoS One       Date:  2016-03-30       Impact factor: 3.240

Review 10.  Biotechnological Trends in Spider and Scorpion Antivenom Development.

Authors:  Andreas Hougaard Laustsen; Mireia Solà; Emma Christine Jappe; Saioa Oscoz; Line Præst Lauridsen; Mikael Engmark
Journal:  Toxins (Basel)       Date:  2016-07-23       Impact factor: 4.546
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