Literature DB >> 9925624

Green fluorescent protein as a reporter to monitor gene expression and food colonization by Aspergillus flavus.

W Du1, Z Huang, J E Flaherty, K Wells, G A Payne.   

Abstract

Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants.

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Year:  1999        PMID: 9925624      PMCID: PMC91103     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  21 in total

1.  Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum.

Authors:  P Fey; K Compton; E C Cox
Journal:  Gene       Date:  1995-11-07       Impact factor: 3.688

2.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal:  Science       Date:  1994-02-11       Impact factor: 47.728

3.  Overexpression of aflR Leads to Upregulation of Pathway Gene Transcription and Increased Aflatoxin Production in Aspergillus flavus.

Authors:  J E Flaherty; G A Payne
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

4.  Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus.

Authors:  G A Payne; G J Nystrom; D Bhatnagar; T E Cleveland; C P Woloshuk
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

5.  A beta-glucuronidase reporter gene construct for monitoring aflatoxin biosynthesis in Aspergillus flavus.

Authors:  J E Flaherty; M A Weaver; G A Payne; C P Woloshuk
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

6.  Genetics and physiology of aflatoxin biosynthesis.

Authors:  G A Payne; M P Brown
Journal:  Annu Rev Phytopathol       Date:  1998       Impact factor: 13.078

7.  Corn Seed Proteins Inhibitory to Aspergillus flavus and Aflatoxin Biosynthesis.

Authors:  Z Huang; D G White; G A Payne
Journal:  Phytopathology       Date:  1997-06       Impact factor: 4.025

8.  Genetic transformation system for the aflatoxin-producing fungus Aspergillus flavus.

Authors:  C P Woloshuk; E R Seip; G A Payne; C R Adkins
Journal:  Appl Environ Microbiol       Date:  1989-01       Impact factor: 4.792

9.  Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis.

Authors:  C P Woloshuk; K R Foutz; J F Brewer; D Bhatnagar; T E Cleveland; G A Payne
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792

10.  Ranking animal carcinogens: a proposed regulatory approach.

Authors:  R A Squire
Journal:  Science       Date:  1981-11-20       Impact factor: 47.728

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  6 in total

Review 1.  Green fluorescent protein is lighting up fungal biology.

Authors:  J M Lorang; R P Tuori; J P Martinez; T L Sawyer; R S Redman; J A Rollins; T J Wolpert; K B Johnson; R J Rodriguez; M B Dickman; L M Ciuffetti
Journal:  Appl Environ Microbiol       Date:  2001-05       Impact factor: 4.792

2.  Early events in the Fusarium verticillioides-maize interaction characterized by using a green fluorescent protein-expressing transgenic isolate.

Authors:  Liat Oren; Smadar Ezrati; David Cohen; Amir Sharon
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

3.  Development of a GFP-expressing Aspergillus flavus strain to study fungal invasion, colonization, and resistance in cottonseed.

Authors:  Kanniah Rajasekaran; Jeffrey W Cary; Peter J Cotty; Thomas E Cleveland
Journal:  Mycopathologia       Date:  2008-02-12       Impact factor: 2.574

Review 4.  Developing resistance to aflatoxin in maize and cottonseed.

Authors:  Jeffrey W Cary; Kanniah Rajasekaran; Robert L Brown; Meng Luo; Zhi-Yuan Chen; Deepak Bhatnagar
Journal:  Toxins (Basel)       Date:  2011-06-21       Impact factor: 4.546

Review 5.  Prospects for reducing fumonisin contamination of maize through genetic modification.

Authors:  J Duvick
Journal:  Environ Health Perspect       Date:  2001-05       Impact factor: 9.031

6.  Aspergillus flavus Exploits Maize Kernels Using an "Orphan" Secondary Metabolite Cluster.

Authors:  Ludovica Antiga; Sonia Roberta La Starza; Cecilia Miccoli; Simone D'Angeli; Valeria Scala; Marco Zaccaria; Xiaomei Shu; Gregory Obrian; Marzia Beccaccioli; Gary A Payne; Massimo Reverberi
Journal:  Int J Mol Sci       Date:  2020-11-03       Impact factor: 5.923

  6 in total

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