MAP2 expression in developing dendrites of human brainstem auditory neurons.

Immunostaining of cytoskeletal elements has proved to be a useful technique for tracing ontogenetic development in the human central auditory system. In the present study, dendritic development in brainstem auditory nuclei (dorsal and ventral cochlear nuclei, medial and lateral superior olivary nuclei, and inferior colliculus) was studied using an antibody to a microtubule-associated protein, MAP2, a molecule which stabilizes dendritic processes by promoting assembly of microtubules. At 21-22 weeks of gestation, cells within the auditory nuclei first demonstrate cytoplasmic MAP2 immunoreactivity, but no dendritic structures have formed. Filamentous background staining at this stage may represent immunoreactivity in astrocytic processes. By the 24th fetal week, somata of auditory neurons are strongly immunostained and have developed short dendritic processes. During the perinatal period, dendrites extend up to 100-120 microm in length but are still sparsely branched and lack terminal formations. By the sixth postnatal month, neurons in all auditory nuclei have acquired dendritic arbors with a mature appearance. Thus MAP2 immunohistochemistry demonstrates that dendrogenesis in human brainstem auditory nuclei begins 16 weeks prior to term birth but does not reach the stage of mature dendritic morphology until several months into the postnatal period. This extended course of development implies a significant period of time during which neuronal activity could influence dendritic structure and function.