BACKGROUND: There is a great need to learn more about porcine islet physiology because porcine islets represent a promising source of xenogeneic tissue for beta-cell replacement therapy in humans. METHODS: We evaluated the effects of two important physiological regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++]i) by adult porcine islet cells. RESULTS: Exposure to GLP-1 and PACAP significantly potentiated glucose-induced insulin release and improved the sensitivity to glucose as a secretagogue. About 70% of cells stimulated with 20 mmol/L glucose alone showed an increase in [Ca++]i, whereas the addition of GLP-1 and PACAP induced [Ca++]i increases in 86% and 93% of cells, respectively. CONCLUSIONS: The good insulin and [Ca++]i responsiveness of porcine islet cells to both GLP-1 and PACAP provides an additional proof of their suitability for transplantation.
BACKGROUND: There is a great need to learn more about porcine islet physiology because porcine islets represent a promising source of xenogeneic tissue for beta-cell replacement therapy in humans. METHODS: We evaluated the effects of two important physiological regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++]i) by adult porcine islet cells. RESULTS: Exposure to GLP-1 and PACAP significantly potentiated glucose-induced insulin release and improved the sensitivity to glucose as a secretagogue. About 70% of cells stimulated with 20 mmol/L glucose alone showed an increase in [Ca++]i, whereas the addition of GLP-1 and PACAP induced [Ca++]i increases in 86% and 93% of cells, respectively. CONCLUSIONS: The good insulin and [Ca++]i responsiveness of porcine islet cells to both GLP-1 and PACAP provides an additional proof of their suitability for transplantation.
Authors: F Jamen; K Persson; G Bertrand; N Rodriguez-Henche; R Puech; J Bockaert; B Ahrén; P Brabet Journal: J Clin Invest Date: 2000-05 Impact factor: 14.808
Authors: Amy C Kelly; Leah V Steyn; Jenna P Kitzmann; Miranda J Anderson; Kate R Mueller; Nathaniel J Hart; Ronald M Lynch; Klearchos K Papas; Sean W Limesand Journal: Xenotransplantation Date: 2014-05-07 Impact factor: 3.907
Authors: Jessica A Hill; Marta Szabat; Corinne A Hoesli; Blair K Gage; Yu Hsuan C Yang; David E Williams; Michael J Riedel; Dan S Luciani; Tatyana B Kalynyak; Kevin Tsai; Ziliang Ao; Raymond J Andersen; Garth L Warnock; James M Piret; Timothy J Kieffer; James D Johnson Journal: PLoS One Date: 2010-09-23 Impact factor: 3.240