Synchronization of glucose-induced Ca2+ transients in pancreatic beta-cells by a diffusible factor.

Glucose is known to induce transients of cytoplasmic Ca2+ by mobilizing intracellular stores when pancreatic beta-cells are exposed to glucagon. Dual wavelength microfluorometry with fura-2 was used to study such transients in individual beta-cells isolated from ob/ob-mice. The Ca2+ transients were often synchronized in beta-cells situated up to 80 microm apart. The messenger might be nitric oxide, as indicated from a decreased number of synchronized transients in the presence of 500 micromol/l oxyhemoglobin or 10 mmol/l Nomega-nitro-L-arginine methyl ester. The discovery that Ca2+ transients are synchronized in the absence of cell contact indicates the involvement of a diffusible factor in coordinating the activity of the insulin-releasing beta-cells. Copyright 1999 Academic Press.