Literature DB >> 9918334

Formation of virus-like particles when the polyprotein gene (segment A) of infectious bursal disease virus is expressed in insect cells.

F S Kibenge1, B Qian, E Nagy, J R Cleghorn, D Wadowska.   

Abstract

The baculovirus expression vector system was used to examine the expression of the full-length infectious bursal disease virus (IBDV) segment A cDNA, which encodes the structural proteins in a polyprotein precursor that is autocatalytically cleaved to VPX, VP3, and VP4. No VP2 was observed in lysates of recombinant baculovirus infected cells indicating the lack of processing of VPX to VP2 in this system. Virus-like particles (VLP) were purified from the infected insect cells, and on negative staining electron microscopy, looked very similar to authentic IBDV particles in shape and size, suggesting that processing of VPX to VP2 is not necessary for capsid assembly.

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Year:  1999        PMID： 9918334      PMCID： PMC1189515     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  21 in total

Review 1.  Biochemistry and immunology of infectious bursal disease virus.

Authors:  F S Kibenge; A S Dhillon; R G Russell
Journal:  J Gen Virol       Date:  1988-08       Impact factor: 3.891

2.  Birnavirus precursor polyprotein is processed in Escherichia coli by its own virus-encoded polypeptide.

Authors:  M N Jagadish; V J Staton; P J Hudson; A A Azad
Journal:  J Virol       Date:  1988-03       Impact factor: 5.103

3.  Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus.

Authors:  A A Azad; M N Jagadish; M A Brown; P J Hudson
Journal:  Virology       Date:  1987-11       Impact factor: 3.616

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Authors:  F S Kibenge; D J Jackwood; C C Mercado
Journal:  J Gen Virol       Date:  1990-03       Impact factor: 3.891

5.  Synthesis of bluetongue virus (BTV) corelike particles by a recombinant baculovirus expressing the two major structural core proteins of BTV.

Authors:  T J French; P Roy
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

6.  Growth of serotypes I and II and variant strains of infectious bursal disease virus in Vero cells.

Authors:  F S Kibenge; A S Dhillon; R G Russell
Journal:  Avian Dis       Date:  1988 Apr-Jun       Impact factor: 1.577

7.  A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens.

Authors:  K J Fahey; K Erny; J Crooks
Journal:  J Gen Virol       Date:  1989-06       Impact factor: 3.891

8.  Genome cloning and analysis of the large RNA segment (segment A) of a naturally avirulent serotype 2 infectious bursal disease virus.

Authors:  F S Kibenge; P K McKenna; J K Dybing
Journal:  Virology       Date:  1991-09       Impact factor: 3.616

9.  Differential detection of infectious bursal disease virus serotypes, using cDNA probes to VP2 coding region.

Authors:  F S Kibenge
Journal:  Am J Vet Res       Date:  1992-08       Impact factor: 1.156

10.  Bacterial luciferase produced with rapid-screening baculovirus vectors is a sensitive reporter for infection of insect cells and larvae.

Authors:  C D Richardson; M Banville; M Lalumière; J Vialard; E A Meighen
Journal:  Intervirology       Date:  1992       Impact factor: 1.763
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7.  Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4.

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Authors:  Polly Roy; Rob Noad
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