J F McAnulty1. 1. Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA.
Abstract
OBJECTIVE: To determine effects of various storage methods on ex vivo viability of cancellous bone fragments. SAMPLE POPULATION: Cancellous bone fragments obtained from 4 New Zealand White rabbits. PROCEDURE: Cancellous bone fragments were stored for 3 hours on ice in 1 of 5 preservation solutions or in 0.9% NaCl. Fragments were then reperfused (37 C) in oxygenated physiologic buffer solution for 1 hour. Cellular viability in fragments was assessed by ethidium monoazide labeling and fluorescence microscopy. RESULTS: Viability in fresh cancellous bone ranged from 85 to 100% (mean+/-SEM, 88+/-7%). Storage of fragments significantly reduced viability. Viability in bone fragments stored at 22 C in blood-soaked sponges or 0.9% NaCl solution was 63.8+/-3 and 65.2+/-7%, respectively. Use of cold 0.9% NaCI solution reduced viability to 53.6+/-3%. Viability was significantly better for fragments stored in cold phosphate-buffered sucrose (70.4+/-2%) and EuroCollins (71.4+/-3%) solutions. After warm reperfusion, viability was best for fragments stored in cold phosphate-buffered sucrose (70.2+/-4%), EuroCollins (72.6+/-3%), or UW lactobionate solutions (69.6+/-3%), compared with those stored in cold 0.9% NaCl (47.6+/-3%) or hypertonic citrate (53.0+/-3%) solutions or blood-soaked sponges (57.2+/-3%). CONCLUSIONS: Hypothermic storage in solutions designed to prevent temperature-dependent cell injury were best for maintaining cancellous bone fragment viability. CLINICAL RELEVANCE: Hypothermia may be advantageous for use in storing cancellous bone fragments during procedures that dictate a prolonged period between harvest and placement of graft fragments.
OBJECTIVE: To determine effects of various storage methods on ex vivo viability of cancellous bone fragments. SAMPLE POPULATION: Cancellous bone fragments obtained from 4 New Zealand White rabbits. PROCEDURE: Cancellous bone fragments were stored for 3 hours on ice in 1 of 5 preservation solutions or in 0.9% NaCl. Fragments were then reperfused (37 C) in oxygenated physiologic buffer solution for 1 hour. Cellular viability in fragments was assessed by ethidium monoazide labeling and fluorescence microscopy. RESULTS: Viability in fresh cancellous bone ranged from 85 to 100% (mean+/-SEM, 88+/-7%). Storage of fragments significantly reduced viability. Viability in bone fragments stored at 22 C in blood-soaked sponges or 0.9% NaCl solution was 63.8+/-3 and 65.2+/-7%, respectively. Use of cold 0.9% NaCI solution reduced viability to 53.6+/-3%. Viability was significantly better for fragments stored in cold phosphate-buffered sucrose (70.4+/-2%) and EuroCollins (71.4+/-3%) solutions. After warm reperfusion, viability was best for fragments stored in cold phosphate-buffered sucrose (70.2+/-4%), EuroCollins (72.6+/-3%), or UW lactobionate solutions (69.6+/-3%), compared with those stored in cold 0.9% NaCl (47.6+/-3%) or hypertoniccitrate (53.0+/-3%) solutions or blood-soaked sponges (57.2+/-3%). CONCLUSIONS: Hypothermic storage in solutions designed to prevent temperature-dependent cell injury were best for maintaining cancellous bone fragment viability. CLINICAL RELEVANCE: Hypothermia may be advantageous for use in storing cancellous bone fragments during procedures that dictate a prolonged period between harvest and placement of graft fragments.