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Literature DB >> 9914251

The mechanism of action of T7 DNA polymerase.

Abstract

The recent crystal structure determination of T7 DNA polymerase complexed to a deoxynucleoside triphosphate and primer-template DNA has provided the first glimpse of a replicative DNA polymerase in a catalytic complex. The structure complements many functional and structural studies of this and other DNA polymerases, allowing a detailed evaluation of proposals for the mechanism of nucleotidyl transfer and the exploration of the basis for the high fidelity of template-directed DNA synthesis.

Entities: Chemical

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Year: 1998 PMID： 9914251 DOI： 10.1016/s0959-440x(98)80089-4

Source DB: PubMed Journal: Curr Opin Struct Biol ISSN： 0959-440X Impact factor: 6.809

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Cited

72 in total

1. Mapping of ATP binding regions in poly(A) polymerases by photoaffinity labeling and by mutational analysis identifies a domain conserved in many nucleotidyltransferases.

Authors: G Martin; P Jenö; W Keller
Journal: Protein Sci Date: 1999-11 Impact factor: 6.725

2. Replication by a single DNA polymerase of a stretched single-stranded DNA.

Authors: B Maier; D Bensimon; V Croquette
Journal: Proc Natl Acad Sci U S A Date: 2000-10-24 Impact factor: 11.205

3. Tuning DNA "strings": modulating the rate of DNA replication with mechanical tension.

Authors: A Goel; M D Frank-Kamenetskii; T Ellenberger; D Herschbach
Journal: Proc Natl Acad Sci U S A Date: 2001-07-10 Impact factor: 11.205

4. Tuning and switching a DNA polymerase motor with mechanical tension.

Authors: Anita Goel; R Dean Astumian; Dudley Herschbach
Journal: Proc Natl Acad Sci U S A Date: 2003-07-17 Impact factor: 11.205

5. Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.

Authors: Luis G Brieba; Brandt F Eichman; Robert J Kokoska; Sylvie Doublié; Tom A Kunkel; Tom Ellenberger
Journal: EMBO J Date: 2004-08-05 Impact factor: 11.598

6. Orchestration of cooperative events in DNA synthesis and repair mechanism unraveled by transition path sampling of DNA polymerase beta's closing.

Authors: Ravi Radhakrishnan; Tamar Schlick
Journal: Proc Natl Acad Sci U S A Date: 2004-04-06 Impact factor: 11.205

The mechanism of action of T7 DNA polymerase.

1. Mapping of ATP binding regions in poly(A) polymerases by photoaffinity labeling and by mutational analysis identifies a domain conserved in many nucleotidyltransferases.

2. Replication by a single DNA polymerase of a stretched single-stranded DNA.

3. Tuning DNA "strings": modulating the rate of DNA replication with mechanical tension.

4. Tuning and switching a DNA polymerase motor with mechanical tension.

5. Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.

6. Orchestration of cooperative events in DNA synthesis and repair mechanism unraveled by transition path sampling of DNA polymerase beta's closing.

7. Kinetic analysis of 3'-5' nucleotide addition catalyzed by eukaryotic tRNA(His) guanylyltransferase.

8. Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.

9. A unique error signature for human DNA polymerase nu.

10. Mismatched base-pair simulations for ASFV Pol X/DNA complexes help interpret frequent G*G misincorporation.