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Literature DB >> 990393

A method for measuring picosecond phenomena in photolabile species: the emission lifetime of bacteriorhodopsin.

M D Hirsch, M A Marcus, A Lewis, H Mahr, N Frigo.

Abstract

We have measured the emission lifetime of bacteriorhodopsin at physiological temperatures to be 15 +/- 3 ps using a technique which employs a mode-locked dye laser, a sum frequency light gate, and a continuous flow system. We observe no concentration dependence of the lifetime over the range of 1.1 X 10(-4) M to 1.0 X 10(-5) M. We conclude that the emission which we observe comes from bacteriorhodopsin and not one of its photochemically produced intermediates, and that the emission cannot originate from the state into which light is absorbed.

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Year: 1976 PMID： 990393 PMCID： PMC1334971 DOI： 10.1016/S0006-3495(76)85783-9

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

19 in total

1. A natural CD label to probe the structure of the purple membrane from Halobacterium halobium by means of exciton coupling effects.

Authors: M P Heyn; P J Bauer; N A Dencher
Journal: Biochem Biophys Res Commun Date: 1975-12-01 Impact factor: 3.575

2. Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium.

Authors: R H Lozier; R A Bogomolni; W Stoeckenius
Journal: Biophys J Date: 1975-09 Impact factor: 4.033

3. Picosecond exciton annihilation in photosynthetic systems.

Authors: A J Campillo; S L Shapiro; V H Kollman; K R Winn; R C Hyer
Journal: Biophys J Date: 1976-01 Impact factor: 4.033

4. Multiple excitations in photosynthetic systems.

Authors: D Mauzerall
Journal: Biophys J Date: 1976-01 Impact factor: 4.033

5. Observation of light emission from a rhodopsin.

Authors: A Lewis; J P Spoonhower; G J Perreault
Journal: Nature Date: 1976-04-22 Impact factor: 49.962

6. Primary photochemical processes in bacteriorhodopsin.

Authors: K J Kaufmann; P M Rentzepis; W Stoeckenius; A Lewis
Journal: Biochem Biophys Res Commun Date: 1976-02-23 Impact factor: 3.575

7. Evidence for chromophore-chromophore (exciton) interaction in the purple membrane of Halobacterium halobium.

Authors: B Becher; T G Ebrey
Journal: Biochem Biophys Res Commun Date: 1976-03-08 Impact factor: 3.575

8. Molecular structure determination by electron microscopy of unstained crystalline specimens.

Authors: P N Unwin; R Henderson
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

9. Picosecond kinetics of the fluorescence from the chromophore of the purple membrane protein of Halobacterium halobium.

Authors: R R Alfano; R Govindjee; B Becher; T G Ebrey
Journal: Biophys J Date: 1976-05 Impact factor: 4.033

10. Light-induced glutamate transport in Halobacterium halobium envelope vesicles. I. Kinetics of the light-dependent and the sodium-gradient-dependent uptake.

Authors: J K Lanyi; V Yearwood-Drayton; R E MacDonald
Journal: Biochemistry Date: 1976-04-20 Impact factor: 3.162

7 in total

A method for measuring picosecond phenomena in photolabile species: the emission lifetime of bacteriorhodopsin.

1. A natural CD label to probe the structure of the purple membrane from Halobacterium halobium by means of exciton coupling effects.

2. Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium.

3. Picosecond exciton annihilation in photosynthetic systems.

4. Multiple excitations in photosynthetic systems.

5. Observation of light emission from a rhodopsin.

6. Primary photochemical processes in bacteriorhodopsin.

7. Evidence for chromophore-chromophore (exciton) interaction in the purple membrane of Halobacterium halobium.

8. Molecular structure determination by electron microscopy of unstained crystalline specimens.

9. Picosecond kinetics of the fluorescence from the chromophore of the purple membrane protein of Halobacterium halobium.

10. Light-induced glutamate transport in Halobacterium halobium envelope vesicles. I. Kinetics of the light-dependent and the sodium-gradient-dependent uptake.

1. Early picosecond events in the photocycle of bacteriorhodopsin.

2. Primary photophysical and photochemical processes in visual excitation.

3. The molecular mechanism of excitation in visual transduction and bacteriorhodopsin.

4. Excited-state dynamics of bacteriorhodopsin.

5. Picosecond and steady state, variable intensity and variable temperature emission spectroscopy of bacteriorhodopsin.

6. Kinetics of the 580-nm ultrafast bacteriorhodopsin transient.

7. The fluorescence from the chromophore of the purple membrane protein.