H Nakano1. 1. Department of Orthopaedic Surgery, Teikyo University School of Medicine, Tokyo, Japan.
Abstract
BACKGROUND: In 1952, Christopherson et al proposed that alveolar soft part sarcoma (ASPS) was a distinct entity with unique clinical and pathological features. Since their report, however, the histogenesis has remained undetermined. MATERIALS AND METHODS: In order to investigate the histogenesis of ASPS, two cases were examined using the immunofluorescence antibody (IF) method, the avidin-biotin peroxidase complex (ABC) method, Western bloffing with an anti-actin polyclonal antibody, and reverse transcription polymerase chain reaction (RT-PCR). Moreover, a total of seven cases were examined using the ABC method involving eight antibodies related to skeletal muscle; RESULTS: The results obtained from the IF method suggested that the granules in the cytoplasm were probably actin. The results from the IF method, Western blotting and RT-PCR were compatible with the presence of alpha-actin from human skeletal muscle in ASPS, while the ABC method using eight muscle-related antibodies also suggested the origin of ASPS was skeletal muscle. CONCLUSION: It is postulated that the histogenesis of ASPS is of skeletal muscle origin.
BACKGROUND: In 1952, Christopherson et al proposed that alveolar soft part sarcoma (ASPS) was a distinct entity with unique clinical and pathological features. Since their report, however, the histogenesis has remained undetermined. MATERIALS AND METHODS: In order to investigate the histogenesis of ASPS, two cases were examined using the immunofluorescence antibody (IF) method, the avidin-biotin peroxidase complex (ABC) method, Western bloffing with an anti-actin polyclonal antibody, and reverse transcription polymerase chain reaction (RT-PCR). Moreover, a total of seven cases were examined using the ABC method involving eight antibodies related to skeletal muscle; RESULTS: The results obtained from the IF method suggested that the granules in the cytoplasm were probably actin. The results from the IF method, Western blotting and RT-PCR were compatible with the presence of alpha-actin from human skeletal muscle in ASPS, while the ABC method using eight muscle-related antibodies also suggested the origin of ASPS was skeletal muscle. CONCLUSION: It is postulated that the histogenesis of ASPS is of skeletal muscle origin.