Cytochemical observations on the extracellular carbohydrate produced by Streptococcus cremoris.

Electron microscopy showed that a varying proportion of cells of Streptococcus cremoris NCDO 924 grown in autoclaved skim-milk possessed a layer of extracellular material attached to the cell wall. Occasional filamentous extensions of this layer made contact with neighbouring casein micelles. The same surface-staining material persisted during the production of cheese-curd, but after maximum scald it was predominantly filamentous in appearance. These filaments made frequent contact with the adjacent curd matrix and with milk-fat globules. Str. cremoris NCDO 1986 produced similar surface material when in curd, but not when grown in skim-milk. In all situations, the extracellular material stained with colloidal iron hydroxide, ruthenium red and periodic acid-thiosemicarbazide-silver proteinate, indicating that it was largely composed of an acidic carbohydrate. It is suggested that this carbohydrate facilitates the adhesion of starter bacteria to the cheese-curd matrix and that during the initial stages of syneresis this serves to prevent their expulsion from the curd with the whey.