Time domain reflectometry: measurement of free water in normal lung and pulmonary edema.

The free water content of lung tissue was investigated by dielectric spectroscopy in normal lungs and in pulmonary edema induced by oleic acid in rats. The dielectric relaxation in a frequency range of 10(7) to 10(10) Hz was measured with the time domain reflectometry method at 25 degreesC. Three dielectric relaxation processes were analyzed for the lung tissue. A high-frequency process around 10 GHz was attributed to the orientation of free water molecules based on the relaxation time [log tauh (in s) = -11.03]. The dielectric strength (Delta epsilon) of this high-frequency peak (Delta epsilonh) should reflect the amount of free water in the tissue. Because the measured Delta epsilonh depended on the air content of the lung samples, the value of Delta epsilonh was corrected for the air content of each sample as determined by the point-counting method in the area where the time domain reflectometry data were obtained. The lungs of rats that received an injection of oleic acid had a significantly increased free water content [(Delta epsilonh of lung/Delta epsilon of pure water) x density of pure water] compared with that in the normal lung (0.76 vs. 0.59 g/cm3). These results indicate that free water occupies approximately 60% of the total volume of normal lung tissue and that there is an increase in free water in pulmonary edema.