Literature DB >> 9885567

Distal sequences, but not ori-beta/OBR-1, are essential for initiation of DNA replication in the Chinese hamster DHFR origin.

R F Kalejta1, X Li, L D Mesner, P A Dijkwel, H B Lin, J L Hamlin.   

Abstract

In the Chinese hamster dihydrofolate reductase replication initiation zone, the ori-beta locus is preferred over other start sites. To test the hypothesis that ori-beta contains a genetic replicator, we restored a deletion in the 3' end of the DHFR gene with a cosmid that provides the missing sequence and simultaneously knocks out the downstream ori-beta locus. Replication initiates normally in ori-beta knockout cell lines, and the DHFR domain is still synthesized in early S phase. However, initiation is completely suppressed in the starting deletion variant lacking the 3' end of the gene. We conclude that ori-beta does not contain an essential replicator, but that distant sequence elements have profound effects on origin activity in this locus.

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Year:  1998        PMID： 9885567     DOI： 10.1016/s1097-2765(00)80294-4

Source DB:  PubMed          Journal:  Mol Cell        ISSN： 1097-2765            Impact factor:   17.970


  46 in total

1.  Functionally distinct, sequence-specific replicator and origin elements are required for Drosophila chorion gene amplification.

Authors:  L Lu; H Zhang; J Tower
Journal:  Genes Dev       Date:  2001-01-15       Impact factor: 11.361

2.  Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.

Authors:  D M Cimbora; D Schübeler; A Reik; J Hamilton; C Francastel; E M Epner; M Groudine
Journal:  Mol Cell Biol       Date:  2000-08       Impact factor: 4.272

Review 3.  Making sense of eukaryotic DNA replication origins.

Authors:  D M Gilbert
Journal:  Science       Date:  2001-10-05       Impact factor: 47.728

4.  The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.

Authors:  L D Mesner; J L Hamlin; P A Dijkwel
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-10       Impact factor: 11.205

5.  Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.

Authors:  D A Natale; C J Li; W H Sun; M L DePamphilis
Journal:  EMBO J       Date:  2000-06-01       Impact factor: 11.598

6.  Replication of the chicken beta-globin locus: early-firing origins at the 5' HS4 insulator and the rho- and betaA-globin genes show opposite epigenetic modifications.

Authors:  Marie-Noëlle Prioleau; Marie-Claude Gendron; Olivier Hyrien
Journal:  Mol Cell Biol       Date:  2003-05       Impact factor: 4.272

7.  Developmental changes in the Sciara II/9A initiation zone for DNA replication.

Authors:  Victoria V Lunyak; Michael Ezrokhi; Heidi S Smith; Susan A Gerbi
Journal:  Mol Cell Biol       Date:  2002-12       Impact factor: 4.272

8.  Modular structure of the human lamin B2 replicator.

Authors:  Sónia Paixão; Ivan N Colaluca; Matthieu Cubells; Fiorenzo A Peverali; Annarita Destro; Sara Giadrossi; Mauro Giacca; Arturo Falaschi; Silvano Riva; Giuseppe Biamonti
Journal:  Mol Cell Biol       Date:  2004-04       Impact factor: 4.272

Review 9.  In search of the holy replicator.

Authors:  David M Gilbert
Journal:  Nat Rev Mol Cell Biol       Date:  2004-10       Impact factor: 94.444

10.  Unraveling cell type-specific and reprogrammable human replication origin signatures associated with G-quadruplex consensus motifs.

Authors:  Emilie Besnard; Amélie Babled; Laure Lapasset; Ollivier Milhavet; Hugues Parrinello; Christelle Dantec; Jean-Michel Marin; Jean-Marc Lemaitre
Journal:  Nat Struct Mol Biol       Date:  2012-07-01       Impact factor: 15.369
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