Literature DB >> 9881664

Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA.

H Tanaka1, Y Shoyama.   

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3-X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml(-1) to 200 ng ml(-1) of glycyrrhizin.

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Year:  1998        PMID： 9881664     DOI： 10.1248/bpb.21.1391

Source DB:  PubMed          Journal:  Biol Pharm Bull        ISSN： 0918-6158            Impact factor:   2.233


  7 in total

1.  Screening of Glycyrrhiza uralensis Fisch. ex DC. containing high concentrations of glycyrrhizin by Eastern blotting and enzyme-linked immunosorbent assay using anti-glycyrrhizin monoclonal antibody for selective breeding of licorice.

Authors:  Shunsuke Fujii; Indree Tuvshintogtokh; Bayart Mandakh; Battseren Munkhjargal; Takuhiro Uto; Osamu Morinaga; Yukihiro Shoyama
Journal:  J Nat Med       Date:  2014-05-21       Impact factor: 2.343

2.  Production of monoclonal antibodies against antitumor cyclohexapeptide RA-VII from Rubia cordifolia and their characterization.

Authors:  Tomoyo Hasuda; Yukio Hitotsuyanagi; Koichi Takeya
Journal:  J Nat Med       Date:  2011-02-08       Impact factor: 2.343

3.  Infusion of gliotoxins or a gap junction blocker in the prelimbic cortex increases alcohol preference in Wistar rats.

Authors:  J Miguel-Hidalgo; Y Shoyama; V Wanzo
Journal:  J Psychopharmacol       Date:  2008-06-18       Impact factor: 4.153

4.  Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors:  Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal:  Int J Anal Chem       Date:  2012-02-14       Impact factor: 1.885

5.  Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes.

Authors:  Li Zhang; Yang Sun; Ting Chen; Qiang Xu
Journal:  Int Immunopharmacol       Date:  2005-03-21       Impact factor: 4.932

Review 6.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06

Review 7.  Lateral flow immunoassay for small-molecules detection in phytoproducts: a review.

Authors:  Poomraphie Nuntawong; Waraporn Putalun; Hiroyuki Tanaka; Satoshi Morimoto; Seiichi Sakamoto
Journal:  J Nat Med       Date:  2022-02-16       Impact factor: 3.192
  7 in total

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