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Literature DB >> 9873663

Evaluation of resins for on-bead screening: a study of papain and chymotrypsin specificity using PEGA-bound combinatorial peptide libraries.

Abstract

TentaGel, ArgoGel and PEGA resins were evaluated for on-bead biological screening, using a fluorescently-labelled peptide attached to each and assayed for papain activity. Peptide attached to PEGA was cleaved in near quantitative yield at the expected sites, whilst an identical sequence on TentaGel and ArgoGel beads was hydrolysed in very low yields and nonspecifically on ArgoGel. The compatibility of PEGA with enzymes was further demonstrated by the determination of subsite specificities of papain and chymotrypsin using PEGA-bound peptide libraries, which proved to be similar to those observed in free solution.

Entities: Chemical

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Year: 1998 PMID： 9873663 DOI： 10.1016/S0960-894X(98)00534-4

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

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Cited

7 in total

1. Two-Step Enzymatic Modification of Solid-Supported Bergenin in Aqueous and Organic Media.

Authors: Umar Akbar; Dong-Sik Shin; Elizabeth Schneider; Jonathan S Dordick; Douglas S Clark
Journal: Tetrahedron Lett Date: 2010-02-24 Impact factor: 2.415

2. Expanding the Scope of Biocatalysis: Oxidative Biotransformations on Solid-Supported Substrates.

Authors: Sarah J Brooks; Lydie Coulombel; Disha Ahuja; Douglas S Clark; Jonathan S Dordick
Journal: Adv Synth Catal Date: 2008-07-07 Impact factor: 5.837

3. Determination of the substrate specificity of protein-tyrosine phosphatase TULA-2 and identification of Syk as a TULA-2 substrate.

Authors: Xianwen Chen; Lige Ren; Soochong Kim; Nicholas Carpino; James L Daniel; Satya P Kunapuli; Alexander Y Tsygankov; Dehua Pei
Journal: J Biol Chem Date: 2010-07-29 Impact factor: 5.157

7. Correlated matrix-assisted laser desorption/ionization mass spectrometry and fluorescent imaging of photocleavable peptide-coded random bead-arrays.

Authors: Mark J Lim; Ziying Liu; Karen I Braunschweiger; Amany Awad; Kenneth J Rothschild
Journal: Rapid Commun Mass Spectrom Date: 2014-01-15 Impact factor: 2.419

7 in total

Evaluation of resins for on-bead screening: a study of papain and chymotrypsin specificity using PEGA-bound combinatorial peptide libraries.

1. Two-Step Enzymatic Modification of Solid-Supported Bergenin in Aqueous and Organic Media.

2. Expanding the Scope of Biocatalysis: Oxidative Biotransformations on Solid-Supported Substrates.

3. Determination of the substrate specificity of protein-tyrosine phosphatase TULA-2 and identification of Syk as a TULA-2 substrate.

4. Exploration of the one-bead one-compound methodology for the design of prolyl oligopeptidase substrates.

5. Profiling the substrate specificity of protein kinases by on-bead screening of peptide libraries.

6. A general process for the development of peptide-based immunoassays for monoclonal antibodies.

7. Correlated matrix-assisted laser desorption/ionization mass spectrometry and fluorescent imaging of photocleavable peptide-coded random bead-arrays.