Literature DB >> 9871567

A fast and inexpensive method for N-terminal fluorescein-labeling of peptides.

P J Weber1, J E Bader, G Folkers, A G Beck-Sickinger.   

Abstract

A new method has been developed to synthesize fluorescein labeled peptides, compounds of increasing importance in bioorganic chemistry, cell biology, pharmacology, drug targeting and medicinal chemistry. We show, that 4(5)-carboxyfluorescein is much more efficient than the hitherto predominantly utilized reagents 4(5)-carboxyfluorescein-N-succinimidylester and 4(5)-fluoresceinisothiocyanate.

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Year:  1998        PMID: 9871567     DOI: 10.1016/s0960-894x(98)00084-5

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN: 0960-894X            Impact factor:   2.823


  17 in total

1.  Metabolic cleavage of cell-penetrating peptides in contact with epithelial models: human calcitonin (hCT)-derived peptides, Tat(47-57) and penetratin(43-58).

Authors:  Rachel Tréhin; Hanne M Nielsen; Heinz-Georg Jahnke; Ulrike Krauss; Annette G Beck-Sickinger; Hans P Merkle
Journal:  Biochem J       Date:  2004-09-15       Impact factor: 3.857

2.  Cellular internalization of human calcitonin derived peptides in MDCK monolayers: a comparative study with Tat(47-57) and penetratin(43-58).

Authors:  Rachel Tréhin; Ulrike Krauss; Roman Muff; Martina Meinecke; Annette G Beck-Sickinger; Hans P Merkle
Journal:  Pharm Res       Date:  2004-01       Impact factor: 4.200

3.  Antifungal activities of human beta-defensins HBD-1 to HBD-3 and their C-terminal analogs Phd1 to Phd3.

Authors:  Viswanatha Krishnakumari; Nandini Rangaraj; Ramakrishnan Nagaraj
Journal:  Antimicrob Agents Chemother       Date:  2008-09-22       Impact factor: 5.191

4.  The positively charged region of the myosin IIC non-helical tailpiece promotes filament assembly.

Authors:  Daniel Ronen; Masha M Rosenberg; Deborah E Shalev; Michael Rosenberg; Shahar Rotem; Assaf Friedler; Shoshana Ravid
Journal:  J Biol Chem       Date:  2009-12-03       Impact factor: 5.157

5.  Fluorescence polarization assays in high-throughput screening and drug discovery: a review.

Authors:  Matthew D Hall; Adam Yasgar; Tyler Peryea; John C Braisted; Ajit Jadhav; Anton Simeonov; Nathan P Coussens
Journal:  Methods Appl Fluoresc       Date:  2016-04-28       Impact factor: 3.009

6.  Identification of Dekkera bruxellensis (Brettanomyces) from wine by fluorescence in situ hybridization using peptide nucleic acid probes.

Authors:  H Stender; C Kurtzman; J J Hyldig-Nielsen; D Sørensen; A Broomer; K Oliveira; H Perry-O'Keefe; A Sage; B Young; J Coull
Journal:  Appl Environ Microbiol       Date:  2001-02       Impact factor: 4.792

7.  A hybrid cationic peptide composed of human β-defensin-1 and humanized θ-defensin sequences exhibits salt-resistant antimicrobial activity.

Authors:  Sudar Olli; Ramakrishnan Nagaraj; Swapna R Motukupally
Journal:  Antimicrob Agents Chemother       Date:  2014-10-27       Impact factor: 5.191

8.  Fluorescence polarization assays in small molecule screening.

Authors:  Wendy A Lea; Anton Simeonov
Journal:  Expert Opin Drug Discov       Date:  2011-01       Impact factor: 6.098

9.  Inhibition of HLA-DQ2-mediated antigen presentation by analogues of a high affinity 33-residue peptide from alpha2-gliadin.

Authors:  Jiang Xia; Matthew Siegel; Elin Bergseng; Ludvig M Sollid; Chaitan Khosla
Journal:  J Am Chem Soc       Date:  2006-02-15       Impact factor: 15.419

10.  Anionic Polymerization of Methylene Malonate for High-Performance Coatings.

Authors:  Mengfei Huang; Yuan Liu; Guozhen Yang; John Klier; Jessica D Schiffman
Journal:  ACS Appl Polym Mater       Date:  2019-02-21
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