Literature DB >> 9863048

PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type background.

T Lanio1, A Jeltsch.   

Abstract

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Year:  1998        PMID： 9863048     DOI： 10.2144/98256bm06

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


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  2 in total

1.  Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.

Authors:  Joyce Chiu; Paul E March; Ryan Lee; Daniel Tillett
Journal:  Nucleic Acids Res       Date:  2004-12-07       Impact factor: 16.971

2.  Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly.

Authors:  Edson Cárcamo; Abigail Roldán-Salgado; Joel Osuna; Iván Bello-Sanmartin; Jorge A Yáñez; Gloria Saab-Rincón; Héctor Viadiu; Paul Gaytán
Journal:  ACS Omega       Date:  2017-07-05
  2 in total

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