| Literature DB >> 9862438 |
A M Alekseev1, S V Shulga-Morskoy, D V Zinchenko, S A Shulga-Morskaya, D V Suchkov, S A Vaganova, I I Senin, A A Zargarov, V M Lipkin, M Akhtar, P P Philippov.
Abstract
Several EF-hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined. The mutants with the 'spoiled' 2nd, 3rd or (2nd+3rd) EF-hand structures did not act upon the kinase activity in the microM range of Ca2+ concentrations. Mutations of the 4th EF hand, which 'repaired' its Ca2+-binding activity, resulted in recoverin with three 'working' Ca2+-binding sites. The latter mutant inhibited rhodopsin kinase even more effectively than the wild-type recoverin, containing two working Ca2+-binding structures.Entities:
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Year: 1998 PMID： 9862438 DOI： 10.1016/s0014-5793(98)01426-4
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124