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Literature DB >> 9859990

Defining the functions of trans-SNARE pairs.

Abstract

The homotypic fusion of yeast vacuoles includes a 'docking' step, which we show here to consist of two sequential reactions: a reversible 'tethering' mediated by the GTPase Ypt7, and 'SNARE pairing', in which SNARE proteins from opposite membranes form a complex in trans. The function of this trans-SNARE complex must be transient, as the complex can be disassembled by excess Sec18 in the presence of Sec17 and ATP without influencing the fusion rate. These data indicate that SNARE pairing may transiently signal to downstream factors, leading to fusion.

Entities: Chemical Gene Species

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Year: 1998 PMID： 9859990 DOI： 10.1038/25069

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

110 in total

1. Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells.

Authors: T Xu; U Ashery; R D Burgoyne; E Neher
Journal: EMBO J Date: 1999-06-15 Impact factor: 11.598

Review 2. Proteins involved in synaptic vesicle trafficking.

Authors: G J Augustine; M E Burns; W M DeBello; S Hilfiker; J R Morgan; F E Schweizer; H Tokumaru; K Umayahara
Journal: J Physiol Date: 1999-10-01 Impact factor: 5.182

3. Fusion of endosomes involved in synaptic vesicle recycling.

Authors: C Holroyd; U Kistner; W Annaert; R Jahn
Journal: Mol Biol Cell Date: 1999-09 Impact factor: 4.138

4. Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion.

Authors: C Ungermann; W Wickner; Z Xu
Journal: Proc Natl Acad Sci U S A Date: 1999-09-28 Impact factor: 11.205

5. TRAPP stably associates with the Golgi and is required for vesicle docking.

Authors: J Barrowman; M Sacher; S Ferro-Novick
Journal: EMBO J Date: 2000-03-01 Impact factor: 11.598

6. A new class of fusion-associated small transmembrane (FAST) proteins encoded by the non-enveloped fusogenic reoviruses.

Authors: M Shmulevitz; R Duncan
Journal: EMBO J Date: 2000-03-01 Impact factor: 11.598

Defining the functions of trans-SNARE pairs.

1. Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells.

Review 2. Proteins involved in synaptic vesicle trafficking.

3. Fusion of endosomes involved in synaptic vesicle recycling.

4. Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion.

5. TRAPP stably associates with the Golgi and is required for vesicle docking.

6. A new class of fusion-associated small transmembrane (FAST) proteins encoded by the non-enveloped fusogenic reoviruses.

7. The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo.

8. Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs.

9. SNARE proteins mediate lipid bilayer fusion.

10. Sequential action of two GTPases to promote vacuole docking and fusion.