Literature DB >> 9854092

Comparison of two PCRs for detection of Mycobacterium ulcerans.

A Guimaraes-Peres1, F Portaels, P de Rijk, K Fissette, S R Pattyn, J van Vooren, P Fonteyne.   

Abstract

Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.

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Year:  1999        PMID： 9854092      PMCID： PMC84208     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

Review 1.  Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species.

Authors:  V V Lévy-Frébault; F Portaels
Journal:  Int J Syst Bacteriol       Date:  1992-04

2.  Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection.

Authors:  B C Ross; L Marino; F Oppedisano; R Edwards; R M Robins-Browne; P D Johnson
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

3.  Selective isolation of mycobacteria from soil: a statistical analysis approach.

Authors:  F Portaels; A De Muynck; M P Sylla
Journal:  J Gen Microbiol       Date:  1988-03

4.  A method for counting acid-fast bacteria.

Authors:  C C Shepard; D H McRae
Journal:  Int J Lepr Other Mycobact Dis       Date:  1968 Jan-Mar

5.  [Bacteriology and human and experimental pathology of ulcers caused by Mycobacterium ulcerans].

Authors:  S R Pattyn
Journal:  Ann Soc Belges Med Trop Parasitol Mycol       Date:  1965

6.  A new Mycobacterium which caused skin infection.

Authors:  M Tsukamura; H Mikoshiba
Journal:  Microbiol Immunol       Date:  1982       Impact factor: 1.955

7.  Mycobacterium ulcerans infection (Buruli ulcer): first reported patients in Togo.

Authors:  W M Meyers; N Tignokpa; G B Priuli; F Portaels
Journal:  Br J Dermatol       Date:  1996-06       Impact factor: 9.302

8.  Direct detection and identification of Mycobacterium ulcerans in clinical specimens by PCR and oligonucleotide-specific capture plate hybridization.

Authors:  F Portaels; J Agular; K Fissette; P A Fonteyne; H De Beenhouwer; P de Rijk; A Guédénon; R Lemans; C Steunou; C Zinsou; J M Dumonceau; W M Meyers
Journal:  J Clin Microbiol       Date:  1997-05       Impact factor: 5.948

9.  Isolation of Mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective Dubos medium.

Authors:  F Saxegaard
Journal:  J Clin Microbiol       Date:  1985-08       Impact factor: 5.948

Review 10.  [Mycobacterium ulcerans cutaneous infections: Buruli ulcers].

Authors:  R Josse; A Guedenon; H Darie; S Anagonou; F Portaels; W M Meyers
Journal:  Med Trop (Mars)       Date:  1995
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  21 in total

1.  Fine-needle aspiration, an efficient sampling technique for bacteriological diagnosis of nonulcerative Buruli ulcer.

Authors:  Miriam Eddyani; Alexandra G Fraga; Fernando Schmitt; Cécile Uwizeye; Krista Fissette; Christian Johnson; Julia Aguiar; Ghislain Sopoh; Yves Barogui; Wayne M Meyers; Jorge Pedrosa; Françoise Portaels
Journal:  J Clin Microbiol       Date:  2009-04-22       Impact factor: 5.948

2.  A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans.

Authors:  T Stinear; J K Davies; G A Jenkin; F Portaels; B C Ross; F Oppedisano; M Purcell; J A Hayman; P D Johnson
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

3.  Nerve damage in Mycobacterium ulcerans-infected mice: probable cause of painlessness in buruli ulcer.

Authors:  Masamichi Goto; Kazue Nakanaga; Thida Aung; Tomofumi Hamada; Norishige Yamada; Mitsuharu Nomoto; Shinichi Kitajima; Norihisa Ishii; Suguru Yonezawa; Hajime Saito
Journal:  Am J Pathol       Date:  2006-03       Impact factor: 4.307

4.  Dry-reagent-based PCR as a novel tool for laboratory confirmation of clinically diagnosed Mycobacterium ulcerans-associated disease in areas in the tropics where M. ulcerans is endemic.

Authors:  V Siegmund; O Adjei; P Racz; C Berberich; E Klutse; F van Vloten; T Kruppa; B Fleischer; G Bretzel
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

5.  Effects of decontamination, DNA extraction, and amplification procedures on the molecular diagnosis of Mycobacterium ulcerans disease (Buruli ulcer).

Authors:  Dissou Affolabi; N'Dira Sanoussi; Koen Vandelannoote; Mathieu Odoun; Frank Faïhun; Ghislain Sopoh; Séverin Anagonou; Françoise Portaels; Miriam Eddyani
Journal:  J Clin Microbiol       Date:  2012-01-18       Impact factor: 5.948

6.  Primary culture of Mycobacterium ulcerans from human tissue specimens after storage in semisolid transport medium.

Authors:  Miriam Eddyani; Martine Debacker; Anandi Martin; Julia Aguiar; Christian R Johnson; Cécile Uwizeye; Krista Fissette; Françoise Portaels
Journal:  J Clin Microbiol       Date:  2007-11-07       Impact factor: 5.948

7.  Analysis of an IS2404-based nested PCR for diagnosis of Buruli ulcer disease in regions of Ghana where the disease is endemic.

Authors:  Ymkje Stienstra; Tjip S van der Werf; Jeannette Guarner; Pratima L Raghunathan; Ellen A Spotts Whitney; Winette T A van der Graaf; Kwame Asamoa; Jordan W Tappero; David A Ashford; C Harold King
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

8.  Aquatic insects as a vector for Mycobacterium ulcerans.

Authors:  Laurent Marsollier; Raymond Robert; Jacques Aubry; Jean-Paul Saint André; Henri Kouakou; Pierre Legras; Anne-Lise Manceau; Chetaou Mahaza; Bernard Carbonnelle
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

9.  Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these bacteria in the environment.

Authors:  Laurent Marsollier; Timothy Stinear; Jacques Aubry; Jean Paul Saint André; Raymond Robert; Pierre Legras; Anne-Lise Manceau; Christine Audrain; Sandra Bourdon; Henri Kouakou; Bernard Carbonnelle
Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792

10.  Occurrence of free-living amoebae in communities of low and high endemicity for Buruli ulcer in southern Benin.

Authors:  Miriam Eddyani; Johan F De Jonckheere; Lies Durnez; Patrick Suykerbuyk; Herwig Leirs; Françoise Portaels
Journal:  Appl Environ Microbiol       Date:  2008-09-05       Impact factor: 4.792
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