K H Oshima1, T Evans-Strickfaden, A Highsmith. 1. Biological Products Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Atlanta, GA, USA. koshima@NMSU.Edu
Abstract
BACKGROUND AND OBJECTIVES: We examined the ability of a modified polyvinylidene fluoride membrane filter to remove blood-borne and surrogate viruses. MATERIALS AND METHODS: Phages PR772 and PP7, hepatitis B virus (HBV), hepatitis C virus and simian virus 40 (SV40) were spiked in minimum essential media with 10% fetal calf serum and the concentration of these viruses compared before and after filtration by either plaque assay or polymerase chain reaction. RESULTS: Viruses >50 nm were removed to below detection limits (>10(6) logs) for all filters tested. A 5-log reduction of HBV (42 nm) and 2- to 3-log reduction of HCV (30-65 nm) was observed. CONCLUSION: A predictable size-based removal of viral agents was observed. The results also suggest the possible utility of SV40 as a surrogate to HBV for membrane filter challenge studies.
BACKGROUND AND OBJECTIVES: We examined the ability of a modified polyvinylidene fluoride membrane filter to remove blood-borne and surrogate viruses. MATERIALS AND METHODS: Phages PR772 and PP7, hepatitis B virus (HBV), hepatitis C virus and simian virus 40 (SV40) were spiked in minimum essential media with 10% fetal calf serum and the concentration of these viruses compared before and after filtration by either plaque assay or polymerase chain reaction. RESULTS: Viruses >50 nm were removed to below detection limits (>10(6) logs) for all filters tested. A 5-log reduction of HBV (42 nm) and 2- to 3-log reduction of HCV (30-65 nm) was observed. CONCLUSION: A predictable size-based removal of viral agents was observed. The results also suggest the possible utility of SV40 as a surrogate to HBV for membrane filter challenge studies.