Glycated haemoglobin in various pathological conditions: investigations based on a new, fully automated method.

A new automated affinity chromatographic method for determining glycated haemoglobin (GHb) in dogs and cats was tested. The method appeared to be practical, quick and accurate. The reference range, calculated on the basis of 50 healthy dogs and 43 healthy cats, lay between 2.4 and 3.4 per cent in dogs and 2.0 and 2.9 per cent in cats. Concentrations were not influenced by age or gender. GHb levels obtained for 21 dogs and 18 cats with newly diagnosed diabetes mellitus were significantly higher than those of the control animals, ranging from 4.5 to 8.6 per cent (median 6.1) in dogs and from 2.7 to 6.0 per cent (median 3.8 per cent) in cats. The GHb levels in 31 normoglycaemic dogs with anaemia ranged from 2.3 to 4.3 per cent (median 3.3 per cent), and those of 22 normoglycaemic cats with anaemia from 2.6 to 3.9 per cent (median 3.2 per cent); both sets of levels were significantly elevated compared to control group values. GHb concentrations in animals with polycythaemia, azotaemia or liver disease showed no significant deviations from the control group; in individual cases they were slightly elevated compared to the reference range. The automated measuring method employed can be used to determine GHb in dogs and cats. Anaemic animals should generally be excluded from the GHb determination.