Literature DB >> 9841782

Changes in pulsed-field gel electrophoresis patterns in clinical isolates of enterohemorrhagic Escherichia coli O157:H7 associated with loss of Shiga toxin genes.

T Murase1, S Yamai, H Watanabe.   

Abstract

Pulsed-field gel electrophoresis (PFGE) of XbaI-digested DNA fragments of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains showed disappearance of a 70- or 80-kb fragment in their patterns associated with loss of Shiga toxin genes during maintenance or subcultivation. Hybridization experiments with a DNA probe complementary to Shiga toxin sequences revealed that the Shiga toxin genes in the parental strain were located on fragments the same size as the lost fragments from the toxin-negative derivatives. The evidence indicates that PFGE pattern of EHEC O157:H7 may change due to loss of Shiga toxin genes, which is likely to be associated with curing of Shiga toxin gene carrying phages in vitro.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 9841782     DOI： 10.1007/pl00006771

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  20 in total

1.  Genotypic analyses of Escherichia coli O157:H7 and O157 nonmotile isolates recovered from beef cattle and carcasses at processing plants in the Midwestern states of the United States.

Authors:  G A Barkocy-Gallagher; T M Arthur; G R Siragusa; J E Keen; R O Elder; W W Laegreid; M Koohmaraie
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

2.  Polymorphic amplified typing sequences provide a novel approach to Escherichia coli O157:H7 strain typing.

Authors:  Indira T Kudva; Peter S Evans; Nicole T Perna; Timothy J Barrett; G Joel DeCastro; Frederick M Ausubel; Frederick R Blattner; Stephen B Calderwood
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

3.  Effects of repeated subculturing and prolonged storage at room temperature of enterohemorrhagic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profiles.

Authors:  Atsushi Iguchi; Ro Osawa; Junichi Kawano; Akira Shimizu; Jun Terajima; Haruo Watanabe
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

4.  Insertions, deletions, and single-nucleotide polymorphisms at rare restriction enzyme sites enhance discriminatory power of polymorphic amplified typing sequences, a novel strain typing system for Escherichia coli O157:H7.

Authors:  Indira T Kudva; Robert W Griffin; Megan Murray; Manohar John; Nicole T Perna; Timothy J Barrett; Stephen B Calderwood
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

5.  Comparison of a PCR-restriction fragment length polymorphism (PCR-RFLP) assay to pulsed-field gel electrophoresis to determine the effect of repeated subculture and prolonged storage on RFLP patterns of Shiga toxin-producing Escherichia coli O157:H7.

Authors:  Kensuke Shima; Yuluo Wu; Norihiko Sugimoto; Masahiro Asakura; Kazuhiko Nishimura; Shinji Yamasaki
Journal:  J Clin Microbiol       Date:  2006-09-13       Impact factor: 5.948

6.  Precise dissection of an Escherichia coli O157:H7 outbreak by single nucleotide polymorphism analysis.

Authors:  George Turabelidze; Steven J Lawrence; Hongyu Gao; Erica Sodergren; George M Weinstock; Sahar Abubucker; Todd Wylie; Makedonka Mitreva; Nurmohammad Shaikh; Romesh Gautom; Phillip I Tarr
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948

7.  Shiga toxin gene loss and transfer in vitro and in vivo during enterohemorrhagic Escherichia coli O26 infection in humans.

Authors:  Martina Bielaszewska; Rita Prager; Robin Köck; Alexander Mellmann; Wenlan Zhang; Helmut Tschäpe; Phillip I Tarr; Helge Karch
Journal:  Appl Environ Microbiol       Date:  2007-03-30       Impact factor: 4.792

Review 8.  Future perspectives, applications and challenges of genomic epidemiology studies for food-borne pathogens: A case study of Enterohemorrhagic Escherichia coli (EHEC) of the O157:H7 serotype.

Authors:  Mark Eppinger; Thomas A Cebula
Journal:  Gut Microbes       Date:  2014-09-01

9.  Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM.

Authors:  Martina Bielaszewska; Rita Prager; Wenlan Zhang; Alexander W Friedrich; Alexander Mellmann; Helmut Tschäpe; Helge Karch
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

10.  Longitudinal study of fecal shedding of Escherichia coli O157:H7 in feedlot cattle: predominance and persistence of specific clonal types despite massive cattle population turnover.

Authors:  J T LeJeune; T E Besser; D H Rice; J L Berg; R P Stilborn; D D Hancock
Journal:  Appl Environ Microbiol       Date:  2004-01       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.